Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16582 | 5' | -47.7 | NC_004117.1 | + | 57925 | 0.66 | 0.999803 |
Target: 5'- gGACAgCAUCGAGGCgaacgugACCUACGcCUGg -3' miRNA: 3'- gCUGU-GUAGUUUUG-------UGGGUGCuGAUa -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 78823 | 0.66 | 0.999753 |
Target: 5'- aCGACGacggUAUCcacuGGAACGCCUACGACc-- -3' miRNA: 3'- -GCUGU----GUAG----UUUUGUGGGUGCUGaua -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 60906 | 0.66 | 0.999753 |
Target: 5'- uGACGCGUCu-GACugCCGaGAUUGUu -3' miRNA: 3'- gCUGUGUAGuuUUGugGGUgCUGAUA- -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 44070 | 0.66 | 0.999686 |
Target: 5'- cCGACGCAUCGAAACGaagUUCGUGACa-- -3' miRNA: 3'- -GCUGUGUAGUUUUGU---GGGUGCUGaua -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 13469 | 0.67 | 0.999049 |
Target: 5'- gCGACGCaAUCAcguuugcccauAGCACCgCAUGGCUAa -3' miRNA: 3'- -GCUGUG-UAGUu----------UUGUGG-GUGCUGAUa -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 74174 | 0.67 | 0.998836 |
Target: 5'- aCGAuCGCGUCGAAGCGCagCAUGAUg-- -3' miRNA: 3'- -GCU-GUGUAGUUUUGUGg-GUGCUGaua -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 10747 | 0.68 | 0.998285 |
Target: 5'- cCGAUAaagaugugCAAGugACUCGCGACUGUg -3' miRNA: 3'- -GCUGUgua-----GUUUugUGGGUGCUGAUA- -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 61073 | 0.68 | 0.997936 |
Target: 5'- aGACGCGUCAAuGAUugUCACGAg--- -3' miRNA: 3'- gCUGUGUAGUU-UUGugGGUGCUgaua -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 103919 | 0.68 | 0.997936 |
Target: 5'- cCGACACGUCgAAAGCGCCauCGGCc-- -3' miRNA: 3'- -GCUGUGUAG-UUUUGUGGguGCUGaua -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 103847 | 0.68 | 0.997529 |
Target: 5'- gGGCAgAcUCGAAACACCCGucggGACUGUu -3' miRNA: 3'- gCUGUgU-AGUUUUGUGGGUg---CUGAUA- -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 4229 | 0.68 | 0.997057 |
Target: 5'- --cCACAUCAAAACAaUCACGugUAUu -3' miRNA: 3'- gcuGUGUAGUUUUGUgGGUGCugAUA- -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 35571 | 0.68 | 0.996514 |
Target: 5'- -uGCAUAUCGAcgacAACACCgGCGGCUc- -3' miRNA: 3'- gcUGUGUAGUU----UUGUGGgUGCUGAua -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 58879 | 0.69 | 0.995889 |
Target: 5'- aCGGCACAUCAuuaacauaguGGACAgaCACGACa-- -3' miRNA: 3'- -GCUGUGUAGU----------UUUGUggGUGCUGaua -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 32722 | 0.69 | 0.995822 |
Target: 5'- uGACGCAUCAGAuagcacaGCACCaaaguaggcgauCGCGACa-- -3' miRNA: 3'- gCUGUGUAGUUU-------UGUGG------------GUGCUGaua -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 92750 | 0.69 | 0.994367 |
Target: 5'- uGACACGUguc-GCACCUACGGCa-- -3' miRNA: 3'- gCUGUGUAguuuUGUGGGUGCUGaua -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 45913 | 0.7 | 0.988531 |
Target: 5'- aUGAU-CAUCgAAGACGCUCAUGACUAUu -3' miRNA: 3'- -GCUGuGUAG-UUUUGUGGGUGCUGAUA- -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 151560 | 0.71 | 0.978821 |
Target: 5'- ---gGCAUCAAcAACACCUACGACg-- -3' miRNA: 3'- gcugUGUAGUU-UUGUGGGUGCUGaua -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 51292 | 0.71 | 0.978821 |
Target: 5'- aCGACAC-UCGAAGCGCgCACGcAUUAa -3' miRNA: 3'- -GCUGUGuAGUUUUGUGgGUGC-UGAUa -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 137678 | 0.72 | 0.967459 |
Target: 5'- gGGCACA-CAuuACgGCCCACGACUu- -3' miRNA: 3'- gCUGUGUaGUuuUG-UGGGUGCUGAua -5' |
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16582 | 5' | -47.7 | NC_004117.1 | + | 123546 | 0.73 | 0.947923 |
Target: 5'- uCGACGCGUCG--ACACUUACGACc-- -3' miRNA: 3'- -GCUGUGUAGUuuUGUGGGUGCUGaua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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