Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16583 | 5' | -56.7 | NC_004117.1 | + | 77546 | 0.66 | 0.867546 |
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Target: 5'- -cCGAGgacaAUGCCGCGUUGGCgagugcacacagCGcaccaaacauGCCCa -3' miRNA: 3'- aaGCUCa---UACGGCGCAAUCG------------GC----------CGGG- -5' |
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| 16583 | 5' | -56.7 | NC_004117.1 | + | 19319 | 0.67 | 0.835331 |
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Target: 5'- gUCGAGcAUGCCGCGaaguucacgcAGCaggaucguguacgUGGCCCg -3' miRNA: 3'- aAGCUCaUACGGCGCaa--------UCG-------------GCCGGG- -5' |
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| 16583 | 5' | -56.7 | NC_004117.1 | + | 32224 | 0.67 | 0.827827 |
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Target: 5'- gUUGAaacauGUAUugaGCCGCGUUGGC-GGCCa -3' miRNA: 3'- aAGCU-----CAUA---CGGCGCAAUCGgCCGGg -5' |
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| 16583 | 5' | -56.7 | NC_004117.1 | + | 56573 | 0.67 | 0.819322 |
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Target: 5'- -gCGcGUgGUGCCGUGUaucgAGaaCCGGCCCg -3' miRNA: 3'- aaGCuCA-UACGGCGCAa---UC--GGCCGGG- -5' |
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| 16583 | 5' | -56.7 | NC_004117.1 | + | 113617 | 0.68 | 0.782764 |
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Target: 5'- gUCGAGUGUGCCGUcaacgauGUUGuGCCGcaggugaacuuuGCCa -3' miRNA: 3'- aAGCUCAUACGGCG-------CAAU-CGGC------------CGGg -5' |
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| 16583 | 5' | -56.7 | NC_004117.1 | + | 32071 | 1.1 | 0.002051 |
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Target: 5'- uUUCGAGUAUGCCGCGUUAGCCGGCCCa -3' miRNA: 3'- -AAGCUCAUACGGCGCAAUCGGCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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