Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16584 | 5' | -56.6 | NC_004117.1 | + | 45684 | 0.66 | 0.881016 |
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Target: 5'- -cAUCGUGUCGGUGuaUCGGUcaacauguacGUGCCAa -3' miRNA: 3'- gcUAGCACGGCUACc-AGCCG----------UACGGU- -5' |
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| 16584 | 5' | -56.6 | NC_004117.1 | + | 31125 | 0.66 | 0.873202 |
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Target: 5'- uGAUCGUGUaCGGcgGcGUCGGCAucaacaaauccuaUGCCGu -3' miRNA: 3'- gCUAGCACG-GCUa-C-CAGCCGU-------------ACGGU- -5' |
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| 16584 | 5' | -56.6 | NC_004117.1 | + | 114042 | 0.67 | 0.866618 |
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Target: 5'- gCGAU-GUaGCCGuuuGUCGGUGUGCCAc -3' miRNA: 3'- -GCUAgCA-CGGCuacCAGCCGUACGGU- -5' |
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| 16584 | 5' | -56.6 | NC_004117.1 | + | 19348 | 0.67 | 0.859105 |
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Target: 5'- gGAUCGUGUacGUGGcccgaucgcguUCGGCGUcGCCGa -3' miRNA: 3'- gCUAGCACGgcUACC-----------AGCCGUA-CGGU- -5' |
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| 16584 | 5' | -56.6 | NC_004117.1 | + | 154679 | 0.67 | 0.851391 |
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Target: 5'- gGAUCGUucacaaaaaGuuGGUGGcguaaacaGGCGUGCCAg -3' miRNA: 3'- gCUAGCA---------CggCUACCag------CCGUACGGU- -5' |
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| 16584 | 5' | -56.6 | NC_004117.1 | + | 19864 | 0.67 | 0.832919 |
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Target: 5'- cCGGUCGUGCCGAcuucaaagUGGccauucacgauccuUgGGCG-GCCAu -3' miRNA: 3'- -GCUAGCACGGCU--------ACC--------------AgCCGUaCGGU- -5' |
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| 16584 | 5' | -56.6 | NC_004117.1 | + | 43720 | 0.69 | 0.76472 |
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Target: 5'- aGAUCGUGCCGAUc--CGuGUGUGCCc -3' miRNA: 3'- gCUAGCACGGCUAccaGC-CGUACGGu -5' |
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| 16584 | 5' | -56.6 | NC_004117.1 | + | 39122 | 0.7 | 0.676629 |
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Target: 5'- gCGGUCGUGUagaaGAUGGUugugCGGCAccuUGUCAc -3' miRNA: 3'- -GCUAGCACGg---CUACCA----GCCGU---ACGGU- -5' |
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| 16584 | 5' | -56.6 | NC_004117.1 | + | 34358 | 1.09 | 0.002839 |
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Target: 5'- gCGAUCGUGCCGAUGGUCGGCAUGCCAa -3' miRNA: 3'- -GCUAGCACGGCUACCAGCCGUACGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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