Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16586 | 3' | -53.6 | NC_004117.1 | + | 59178 | 0.66 | 0.965583 |
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Target: 5'- --aCGAug-CGUCaGAUGUAAAGGCGCc -3' miRNA: 3'- uaaGCUcgaGCGG-CUGCAUUUCCGCG- -5' |
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| 16586 | 3' | -53.6 | NC_004117.1 | + | 107667 | 0.66 | 0.958585 |
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Target: 5'- aGUUUGAGaugaUgGCgGAgGUGGAGGCGg -3' miRNA: 3'- -UAAGCUCg---AgCGgCUgCAUUUCCGCg -5' |
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| 16586 | 3' | -53.6 | NC_004117.1 | + | 70458 | 0.66 | 0.954743 |
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Target: 5'- uUUCGuGCgcuaacgUGUCGACG---AGGCGCg -3' miRNA: 3'- uAAGCuCGa------GCGGCUGCauuUCCGCG- -5' |
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| 16586 | 3' | -53.6 | NC_004117.1 | + | 27865 | 0.69 | 0.868154 |
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Target: 5'- --gCGGGC-CGCUguGGCGUcaAGAGGUGCa -3' miRNA: 3'- uaaGCUCGaGCGG--CUGCA--UUUCCGCG- -5' |
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| 16586 | 3' | -53.6 | NC_004117.1 | + | 13180 | 0.7 | 0.836282 |
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Target: 5'- gAUUCGuauggcGCUUGCgGugGUAAugccGGCGCu -3' miRNA: 3'- -UAAGCu-----CGAGCGgCugCAUUu---CCGCG- -5' |
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| 16586 | 3' | -53.6 | NC_004117.1 | + | 69162 | 0.7 | 0.819169 |
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Target: 5'- cUUUGGuuauGCUCGgCGACGaguaugugGAAGGCGCa -3' miRNA: 3'- uAAGCU----CGAGCgGCUGCa-------UUUCCGCG- -5' |
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| 16586 | 3' | -53.6 | NC_004117.1 | + | 19371 | 0.75 | 0.549563 |
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Target: 5'- cGUUCG-GCgUCGCCGACGUuGGGGUucGCg -3' miRNA: 3'- -UAAGCuCG-AGCGGCUGCAuUUCCG--CG- -5' |
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| 16586 | 3' | -53.6 | NC_004117.1 | + | 28471 | 0.76 | 0.519402 |
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Target: 5'- -aUCGuuaucGCUCGCCGAUGUAGcguagcccuuGGCGCg -3' miRNA: 3'- uaAGCu----CGAGCGGCUGCAUUu---------CCGCG- -5' |
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| 16586 | 3' | -53.6 | NC_004117.1 | + | 51633 | 1.09 | 0.004921 |
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Target: 5'- cAUUCGAGCUCGCCGACGUAAAGGCGCc -3' miRNA: 3'- -UAAGCUCGAGCGGCUGCAUUUCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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