Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16591 | 5' | -45.6 | NC_004117.1 | + | 51178 | 0.68 | 0.999579 |
Target: 5'- uAGCGAUUUGCgcACAUGUauuuCUGGAAu -3' miRNA: 3'- uUUGCUAAAUGauUGUACG----GGCCUUu -5' |
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16591 | 5' | -45.6 | NC_004117.1 | + | 108066 | 0.72 | 0.988677 |
Target: 5'- -uACGAUUauaaUACUAACAUGCCCa---- -3' miRNA: 3'- uuUGCUAA----AUGAUUGUACGGGccuuu -5' |
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16591 | 5' | -45.6 | NC_004117.1 | + | 97963 | 0.72 | 0.985227 |
Target: 5'- gAAACGugcgcgGCUGAC-UGCCCGGAu- -3' miRNA: 3'- -UUUGCuaaa--UGAUUGuACGGGCCUuu -5' |
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16591 | 5' | -45.6 | NC_004117.1 | + | 112106 | 1.05 | 0.043197 |
Target: 5'- gAAACGAUUUACUAACAUGCCCGGAAAa -3' miRNA: 3'- -UUUGCUAAAUGAUUGUACGGGCCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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