Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16592 | 5' | -45.1 | NC_004117.1 | + | 92387 | 0.66 | 0.999997 |
Target: 5'- aCGUCguaaAAAAUGAUGugGACAu-- -3' miRNA: 3'- aGCAGauc-UUUUACUGCugCUGUuca -5' |
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16592 | 5' | -45.1 | NC_004117.1 | + | 6238 | 0.68 | 0.999942 |
Target: 5'- uUCGUCgcuaaguGAcAAUGACGAcauagccuCGACGGGUg -3' miRNA: 3'- -AGCAGau-----CUuUUACUGCU--------GCUGUUCA- -5' |
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16592 | 5' | -45.1 | NC_004117.1 | + | 129833 | 0.68 | 0.999898 |
Target: 5'- cCGUaggcuGAGAUGACGACGGCAauGGUu -3' miRNA: 3'- aGCAgauc-UUUUACUGCUGCUGU--UCA- -5' |
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16592 | 5' | -45.1 | NC_004117.1 | + | 120517 | 0.69 | 0.999776 |
Target: 5'- aUCGUCaAGAGGAUGuCGGCGcGCGAu- -3' miRNA: 3'- -AGCAGaUCUUUUACuGCUGC-UGUUca -5' |
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16592 | 5' | -45.1 | NC_004117.1 | + | 129680 | 1.09 | 0.039712 |
Target: 5'- cUCGUCUAGAAAAUGACGACGACAAGUc -3' miRNA: 3'- -AGCAGAUCUUUUACUGCUGCUGUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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