Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16594 | 5' | -53.2 | NC_004117.1 | + | 27647 | 0.66 | 0.969121 |
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Target: 5'- aGCUGGUugGUUGGC---AGGACGACa -3' miRNA: 3'- cCGAUCGugUGACCGcguUCUUGCUG- -5' |
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| 16594 | 5' | -53.2 | NC_004117.1 | + | 136288 | 0.66 | 0.962559 |
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Target: 5'- ----cGCAgCACUGGCGCaAAGAuuggucACGGCu -3' miRNA: 3'- ccgauCGU-GUGACCGCG-UUCU------UGCUG- -5' |
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| 16594 | 5' | -53.2 | NC_004117.1 | + | 52318 | 0.67 | 0.956655 |
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Target: 5'- cGCUGGCAcgauauauuagaauuUugUGGCGCc--AACGACu -3' miRNA: 3'- cCGAUCGU---------------GugACCGCGuucUUGCUG- -5' |
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| 16594 | 5' | -53.2 | NC_004117.1 | + | 77782 | 0.67 | 0.950993 |
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Target: 5'- cGGCgcGGUcguugucgACGCUGGCGCA--AugGGCa -3' miRNA: 3'- -CCGa-UCG--------UGUGACCGCGUucUugCUG- -5' |
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| 16594 | 5' | -53.2 | NC_004117.1 | + | 17576 | 0.67 | 0.94208 |
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Target: 5'- gGGUgcgaucGCACACuuUGGaauggaaGCGAGAACGAUa -3' miRNA: 3'- -CCGau----CGUGUG--ACCg------CGUUCUUGCUG- -5' |
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| 16594 | 5' | -53.2 | NC_004117.1 | + | 1043 | 0.68 | 0.932178 |
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Target: 5'- cGCUAGaAgACggGGCGUGAGAuguGCGACg -3' miRNA: 3'- cCGAUCgUgUGa-CCGCGUUCU---UGCUG- -5' |
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| 16594 | 5' | -53.2 | NC_004117.1 | + | 144552 | 0.68 | 0.921273 |
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Target: 5'- ----uGUACAauaaUGGCgGCGAGGACGACg -3' miRNA: 3'- ccgauCGUGUg---ACCG-CGUUCUUGCUG- -5' |
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| 16594 | 5' | -53.2 | NC_004117.1 | + | 37729 | 0.7 | 0.860084 |
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Target: 5'- -aCUAGCGuCACaaUGGCGCGAGGACu-- -3' miRNA: 3'- ccGAUCGU-GUG--ACCGCGUUCUUGcug -5' |
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| 16594 | 5' | -53.2 | NC_004117.1 | + | 97723 | 0.71 | 0.791068 |
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Target: 5'- uGCUAGCGCAUUacuauGCGCA-GGugGACg -3' miRNA: 3'- cCGAUCGUGUGAc----CGCGUuCUugCUG- -5' |
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| 16594 | 5' | -53.2 | NC_004117.1 | + | 135769 | 0.97 | 0.02855 |
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Target: 5'- cGGCUAGCACACUGGCGacAGAACGACg -3' miRNA: 3'- -CCGAUCGUGUGACCGCguUCUUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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