Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16626 | 3' | -49 | NC_004156.1 | + | 161888 | 0.67 | 0.999046 |
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Target: 5'- aUAuAggUAUCACUGGaGUGGGUAGa- -3' miRNA: 3'- -AUuUuuGUAGUGACCaCGCCCGUUgc -5' |
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| 16626 | 3' | -49 | NC_004156.1 | + | 48063 | 0.67 | 0.998303 |
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Target: 5'- -uGAAACAUCAaguaUGcG-GUGGGCGACa -3' miRNA: 3'- auUUUUGUAGUg---AC-CaCGCCCGUUGc -5' |
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| 16626 | 3' | -49 | NC_004156.1 | + | 146202 | 0.68 | 0.995945 |
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Target: 5'- -uGAAAUcgUACUGGaacccguUGUGGGCAAUGu -3' miRNA: 3'- auUUUUGuaGUGACC-------ACGCCCGUUGC- -5' |
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| 16626 | 3' | -49 | NC_004156.1 | + | 179964 | 0.71 | 0.982116 |
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Target: 5'- gGAAAGCAU-ACUGGUGUcgguGGGCAAg- -3' miRNA: 3'- aUUUUUGUAgUGACCACG----CCCGUUgc -5' |
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| 16626 | 3' | -49 | NC_004156.1 | + | 26157 | 0.74 | 0.926637 |
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Target: 5'- ----uACcgCACUGGUGUaGGCAGCGu -3' miRNA: 3'- auuuuUGuaGUGACCACGcCCGUUGC- -5' |
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| 16626 | 3' | -49 | NC_004156.1 | + | 8269 | 1.09 | 0.017756 |
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Target: 5'- gUAAAAACAUCACUGGUGCGGGCAACGg -3' miRNA: 3'- -AUUUUUGUAGUGACCACGCCCGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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