miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16626 3' -49 NC_004156.1 + 161888 0.67 0.999046
Target:  5'- aUAuAggUAUCACUGGaGUGGGUAGa- -3'
miRNA:   3'- -AUuUuuGUAGUGACCaCGCCCGUUgc -5'
16626 3' -49 NC_004156.1 + 48063 0.67 0.998303
Target:  5'- -uGAAACAUCAaguaUGcG-GUGGGCGACa -3'
miRNA:   3'- auUUUUGUAGUg---AC-CaCGCCCGUUGc -5'
16626 3' -49 NC_004156.1 + 146202 0.68 0.995945
Target:  5'- -uGAAAUcgUACUGGaacccguUGUGGGCAAUGu -3'
miRNA:   3'- auUUUUGuaGUGACC-------ACGCCCGUUGC- -5'
16626 3' -49 NC_004156.1 + 179964 0.71 0.982116
Target:  5'- gGAAAGCAU-ACUGGUGUcgguGGGCAAg- -3'
miRNA:   3'- aUUUUUGUAgUGACCACG----CCCGUUgc -5'
16626 3' -49 NC_004156.1 + 26157 0.74 0.926637
Target:  5'- ----uACcgCACUGGUGUaGGCAGCGu -3'
miRNA:   3'- auuuuUGuaGUGACCACGcCCGUUGC- -5'
16626 3' -49 NC_004156.1 + 8269 1.09 0.017756
Target:  5'- gUAAAAACAUCACUGGUGCGGGCAACGg -3'
miRNA:   3'- -AUUUUUGUAGUGACCACGCCCGUUGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.