Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16627 | 3' | -41.2 | NC_004156.1 | + | 20917 | 0.7 | 1 |
Target: 5'- cGGUUAAAGAGUUUGuuuACG-CGaCGCa -3' miRNA: 3'- -CCGAUUUCUUAAACuu-UGUaGUcGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 150235 | 0.66 | 1 |
Target: 5'- uGGCguagGAGGAgguaGUUUGGGAgG-CAGUGUa -3' miRNA: 3'- -CCGa---UUUCU----UAAACUUUgUaGUCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 81168 | 0.66 | 1 |
Target: 5'- -aCUAGGGAcacGUUUGGAu--UCAGCGUc -3' miRNA: 3'- ccGAUUUCU---UAAACUUuguAGUCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 110238 | 0.67 | 1 |
Target: 5'- aGCUAAAGuuUUUGAucgaguACAUCuuGgGCa -3' miRNA: 3'- cCGAUUUCuuAAACUu-----UGUAGu-CgCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 42939 | 0.67 | 1 |
Target: 5'- ---cAAGGGAUUgu--GCAUCGGUGCa -3' miRNA: 3'- ccgaUUUCUUAAacuuUGUAGUCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 58300 | 0.68 | 1 |
Target: 5'- cGCUAAAcguaaagccGAGgcUGAAGCAgcaaAGCGCa -3' miRNA: 3'- cCGAUUU---------CUUaaACUUUGUag--UCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 20484 | 0.68 | 1 |
Target: 5'- cGCUAuGGGucucUUGAAGaguccCAUCAGUGCa -3' miRNA: 3'- cCGAUuUCUua--AACUUU-----GUAGUCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 81476 | 0.68 | 1 |
Target: 5'- aGGCUAGAGuAGUU---GACGUU-GCGCu -3' miRNA: 3'- -CCGAUUUC-UUAAacuUUGUAGuCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 213556 | 0.68 | 1 |
Target: 5'- gGGCUuguacacguuGAGUgUGAGACAguguUCGGUGCc -3' miRNA: 3'- -CCGAuuu-------CUUAaACUUUGU----AGUCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 19576 | 0.69 | 1 |
Target: 5'- uGCUAAAGAGUUgcgu-UAUCAGCa- -3' miRNA: 3'- cCGAUUUCUUAAacuuuGUAGUCGcg -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 214640 | 0.66 | 1 |
Target: 5'- aGCc-AAGug-UUGAuguAGCAUCAGUGCa -3' miRNA: 3'- cCGauUUCuuaAACU---UUGUAGUCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 98949 | 0.67 | 1 |
Target: 5'- aGuCUAGGGGAUUUGGugauUCAGUGUa -3' miRNA: 3'- cC-GAUUUCUUAAACUuuguAGUCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 201543 | 0.67 | 1 |
Target: 5'- -aCUAAAGug---GAAACAUUAGCaGCa -3' miRNA: 3'- ccGAUUUCuuaaaCUUUGUAGUCG-CG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 149303 | 0.68 | 1 |
Target: 5'- uGGCgu-AGAAggUG--GCGUCGGUGUa -3' miRNA: 3'- -CCGauuUCUUaaACuuUGUAGUCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 141828 | 0.68 | 1 |
Target: 5'- uGC-AAAGAGUaccaaGAguAACAUUAGCGCa -3' miRNA: 3'- cCGaUUUCUUAaa---CU--UUGUAGUCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 164373 | 0.69 | 1 |
Target: 5'- cGGCgauGGGggUgcUGAAugAUCcAGUGCc -3' miRNA: 3'- -CCGau-UUCuuAa-ACUUugUAG-UCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 127229 | 0.78 | 0.998799 |
Target: 5'- aGUUGAAGAgcaaaaucuuuauaAUcUGAGGCAUCAGCGUc -3' miRNA: 3'- cCGAUUUCU--------------UAaACUUUGUAGUCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 199253 | 0.78 | 0.998618 |
Target: 5'- aGGUU--GGAGUUUGAaguuGACGUCGGUGUa -3' miRNA: 3'- -CCGAuuUCUUAAACU----UUGUAGUCGCG- -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 129613 | 0.81 | 0.991106 |
Target: 5'- gGGC-GAAGAGUUUGAGuGCGUCAGCu- -3' miRNA: 3'- -CCGaUUUCUUAAACUU-UGUAGUCGcg -5' |
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16627 | 3' | -41.2 | NC_004156.1 | + | 11439 | 1.2 | 0.034752 |
Target: 5'- aGGCUAAAGAAUUUGAAACAUCAGCGCg -3' miRNA: 3'- -CCGAUUUCUUAAACUUUGUAGUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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