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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16627 | 5' | -49.4 | NC_004156.1 | + | 11164 | 0.72 | 0.937188 |
Target: 5'- aAAGCUUUGACgucuggCUUCGCGCuGAUguuUCa- -3' miRNA: 3'- -UUCGAAACUGa-----GAAGCGCG-CUA---AGau -5' |
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16627 | 5' | -49.4 | NC_004156.1 | + | 53423 | 0.73 | 0.932006 |
Target: 5'- cAAGCU-UGACUCUcagcUUGUGCGGUUCg- -3' miRNA: 3'- -UUCGAaACUGAGA----AGCGCGCUAAGau -5' |
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16627 | 5' | -49.4 | NC_004156.1 | + | 11399 | 1.05 | 0.026876 |
Target: 5'- aAAGCUUUGACUCUUCGCGCGAUUCUAc -3' miRNA: 3'- -UUCGAAACUGAGAAGCGCGCUAAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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