Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16629 | 5' | -53.4 | NC_004156.1 | + | 104306 | 0.66 | 0.988602 |
Target: 5'- -uUGCUAGgGACGCUAGagauGUGUaugCCa -3' miRNA: 3'- ucACGGUCgCUGUGAUCg---UACAga-GG- -5' |
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16629 | 5' | -53.4 | NC_004156.1 | + | 142851 | 0.66 | 0.981761 |
Target: 5'- -aUGCaagaAGCGGCACUGGUGUGcgaaCUCa -3' miRNA: 3'- ucACGg---UCGCUGUGAUCGUACa---GAGg -5' |
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16629 | 5' | -53.4 | NC_004156.1 | + | 133481 | 0.67 | 0.977359 |
Target: 5'- --aGgCAGCGACAUUAGCGgaucguaaacUGUUUCa -3' miRNA: 3'- ucaCgGUCGCUGUGAUCGU----------ACAGAGg -5' |
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16629 | 5' | -53.4 | NC_004156.1 | + | 63712 | 0.67 | 0.977359 |
Target: 5'- ---aUCAGUGGCGCUAGUA-GUCUgCCg -3' miRNA: 3'- ucacGGUCGCUGUGAUCGUaCAGA-GG- -5' |
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16629 | 5' | -53.4 | NC_004156.1 | + | 227288 | 0.67 | 0.974886 |
Target: 5'- aAGUGCCAacucUGAUACUAGUgauUGaCUCCa -3' miRNA: 3'- -UCACGGUc---GCUGUGAUCGu--ACaGAGG- -5' |
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16629 | 5' | -53.4 | NC_004156.1 | + | 43400 | 0.67 | 0.971383 |
Target: 5'- gAGUGCUaccaaaaaaacuagAGCGAUgACUAGCAccgCUCCc -3' miRNA: 3'- -UCACGG--------------UCGCUG-UGAUCGUacaGAGG- -5' |
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16629 | 5' | -53.4 | NC_004156.1 | + | 95948 | 0.67 | 0.970527 |
Target: 5'- --gGCCGGUGagaguagcaacuuuaGCACUguccuggagGGCAUGUCUCa -3' miRNA: 3'- ucaCGGUCGC---------------UGUGA---------UCGUACAGAGg -5' |
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16629 | 5' | -53.4 | NC_004156.1 | + | 206245 | 0.68 | 0.951866 |
Target: 5'- cAGUGCCAGU----CUcAGCAaGUCUCCg -3' miRNA: 3'- -UCACGGUCGcuguGA-UCGUaCAGAGG- -5' |
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16629 | 5' | -53.4 | NC_004156.1 | + | 164397 | 0.69 | 0.933795 |
Target: 5'- cAGUGCCAGC---ACUGGUAcccUCUCCa -3' miRNA: 3'- -UCACGGUCGcugUGAUCGUac-AGAGG- -5' |
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16629 | 5' | -53.4 | NC_004156.1 | + | 80737 | 0.69 | 0.923342 |
Target: 5'- gAGUGCCAGCGuCGguaUGGCAUaGUgUCg -3' miRNA: 3'- -UCACGGUCGCuGUg--AUCGUA-CAgAGg -5' |
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16629 | 5' | -53.4 | NC_004156.1 | + | 39533 | 1.13 | 0.003985 |
Target: 5'- cAGUGCCAGCGACACUAGCAUGUCUCCa -3' miRNA: 3'- -UCACGGUCGCUGUGAUCGUACAGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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