Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 16630 | 3' | -48.7 | NC_004156.1 | + | 143257 | 0.66 | 0.999779 |
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Target: 5'- aCgUGCUGGGAGUGCGuCUCg-GCg-- -3' miRNA: 3'- -GgAUGAUUCUCACGUuGAGgaCGauu -5' |
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| 16630 | 3' | -48.7 | NC_004156.1 | + | 159343 | 0.66 | 0.999645 |
|
Target: 5'- aUCUACUAAGAcgucgGUGUAGCUuuuuaaaguuggCCUcGCUGAa -3' miRNA: 3'- -GGAUGAUUCU-----CACGUUGA------------GGA-CGAUU- -5' |
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| 16630 | 3' | -48.7 | NC_004156.1 | + | 130176 | 0.67 | 0.998471 |
|
Target: 5'- aCUGCUGAGGGUGUAcGCUCacaCUGAc -3' miRNA: 3'- gGAUGAUUCUCACGU-UGAGgacGAUU- -5' |
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| 16630 | 3' | -48.7 | NC_004156.1 | + | 39634 | 0.68 | 0.997374 |
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Target: 5'- --aAC-AAGAGUaCAACUCCUGUUAAg -3' miRNA: 3'- ggaUGaUUCUCAcGUUGAGGACGAUU- -5' |
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| 16630 | 3' | -48.7 | NC_004156.1 | + | 210484 | 0.7 | 0.98668 |
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Target: 5'- cCCUGCUGcGGcUGCGugagcgagaacgGCUCCUGCUGc -3' miRNA: 3'- -GGAUGAUuCUcACGU------------UGAGGACGAUu -5' |
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| 16630 | 3' | -48.7 | NC_004156.1 | + | 19794 | 0.77 | 0.809384 |
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Target: 5'- aCCUGCUcGGAGU-CuugauGCUCCUGCUGAg -3' miRNA: 3'- -GGAUGAuUCUCAcGu----UGAGGACGAUU- -5' |
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| 16630 | 3' | -48.7 | NC_004156.1 | + | 39656 | 0.78 | 0.75158 |
|
Target: 5'- aCCUACcAAGAGUGCAACUCCa----- -3' miRNA: 3'- -GGAUGaUUCUCACGUUGAGGacgauu -5' |
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| 16630 | 3' | -48.7 | NC_004156.1 | + | 39716 | 0.86 | 0.396116 |
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Target: 5'- aCCUACUAAGAGcaCAACUCCUGUUAAg -3' miRNA: 3'- -GGAUGAUUCUCacGUUGAGGACGAUU- -5' |
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| 16630 | 3' | -48.7 | NC_004156.1 | + | 39755 | 0.87 | 0.34543 |
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Target: 5'- uCCUGCUAAGAGUGCAACUCCa----- -3' miRNA: 3'- -GGAUGAUUCUCACGUUGAGGacgauu -5' |
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| 16630 | 3' | -48.7 | NC_004156.1 | + | 39791 | 1.1 | 0.016297 |
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Target: 5'- aCCUACUAAGAGUGCAACUCCUGCUAAg -3' miRNA: 3'- -GGAUGAUUCUCACGUUGAGGACGAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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