Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16630 | 5' | -49.2 | NC_004156.1 | + | 143257 | 0.66 | 0.999526 |
Target: 5'- -aCgUGCUGGGAGUGCGuCUCg---- -3' miRNA: 3'- gaGgACGAUUCUCACGUuGAGguuua -5' |
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16630 | 5' | -49.2 | NC_004156.1 | + | 19684 | 0.66 | 0.999101 |
Target: 5'- uCUUgaGCUGAGAGcGCAACgCCAu-- -3' miRNA: 3'- -GAGgaCGAUUCUCaCGUUGaGGUuua -5' |
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16630 | 5' | -49.2 | NC_004156.1 | + | 210456 | 0.68 | 0.995484 |
Target: 5'- gCUCCUGCUGcGGcUGCGGCcucugCCAGAUc -3' miRNA: 3'- -GAGGACGAUuCUcACGUUGa----GGUUUA- -5' |
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16630 | 5' | -49.2 | NC_004156.1 | + | 130171 | 0.68 | 0.994728 |
Target: 5'- aCUCuaCUGCUGAGGGUGUAcGCUCaCAc-- -3' miRNA: 3'- -GAG--GACGAUUCUCACGU-UGAG-GUuua -5' |
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16630 | 5' | -49.2 | NC_004156.1 | + | 39622 | 0.72 | 0.959257 |
Target: 5'- aCUCCUGUUAAGAGUaCAcCUCCu--- -3' miRNA: 3'- -GAGGACGAUUCUCAcGUuGAGGuuua -5' |
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16630 | 5' | -49.2 | NC_004156.1 | + | 39656 | 0.76 | 0.815229 |
Target: 5'- -aCCUaCcAAGAGUGCAACUCCAAAc -3' miRNA: 3'- gaGGAcGaUUCUCACGUUGAGGUUUa -5' |
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16630 | 5' | -49.2 | NC_004156.1 | + | 39791 | 0.78 | 0.717128 |
Target: 5'- -aCCUaCUAAGAGUGCAACUCCu--- -3' miRNA: 3'- gaGGAcGAUUCUCACGUUGAGGuuua -5' |
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16630 | 5' | -49.2 | NC_004156.1 | + | 39757 | 1.08 | 0.018499 |
Target: 5'- aCUCCUGCUAAGAGUGCAACUCCAAAUa -3' miRNA: 3'- -GAGGACGAUUCUCACGUUGAGGUUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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