Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16631 | 3' | -51.4 | NC_004156.1 | + | 135495 | 0.66 | 0.994479 |
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Target: 5'- uACUACUAcUGGUAGUAgUGCUaGUa-- -3' miRNA: 3'- -UGAUGAUcACCAUCAUgACGA-CGgua -5' |
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| 16631 | 3' | -51.4 | NC_004156.1 | + | 39302 | 0.68 | 0.98199 |
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Target: 5'- cACUGCUAGUGuugcUGGcGCUGCUGUUGUc -3' miRNA: 3'- -UGAUGAUCACc---AUCaUGACGACGGUA- -5' |
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| 16631 | 3' | -51.4 | NC_004156.1 | + | 123687 | 0.68 | 0.977471 |
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Target: 5'- gGCUACcAGUGGUGGUAauaucaaUGCCAa -3' miRNA: 3'- -UGAUGaUCACCAUCAUgacg---ACGGUa -5' |
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| 16631 | 3' | -51.4 | NC_004156.1 | + | 68221 | 0.69 | 0.955098 |
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Target: 5'- aGCUGCUcugcgagucugcAGUGcUGGUACUGCUGgCAc -3' miRNA: 3'- -UGAUGA------------UCACcAUCAUGACGACgGUa -5' |
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| 16631 | 3' | -51.4 | NC_004156.1 | + | 191086 | 0.7 | 0.937152 |
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Target: 5'- cACUACUGGUaGUGGcACUGUgGCCAa -3' miRNA: 3'- -UGAUGAUCAcCAUCaUGACGaCGGUa -5' |
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| 16631 | 3' | -51.4 | NC_004156.1 | + | 145446 | 0.71 | 0.915181 |
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Target: 5'- gUUGUUGGUGGUGGUGCUGUUGUUg- -3' miRNA: 3'- uGAUGAUCACCAUCAUGACGACGGua -5' |
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| 16631 | 3' | -51.4 | NC_004156.1 | + | 12294 | 0.72 | 0.882079 |
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Target: 5'- gACUAUgGGUGGUAGaUGCUGUUGgCAg -3' miRNA: 3'- -UGAUGaUCACCAUC-AUGACGACgGUa -5' |
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| 16631 | 3' | -51.4 | NC_004156.1 | + | 53597 | 0.72 | 0.882079 |
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Target: 5'- uACUggcACUGGUGGUGGUGCUgguuuugauGCUGgCAUu -3' miRNA: 3'- -UGA---UGAUCACCAUCAUGA---------CGACgGUA- -5' |
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| 16631 | 3' | -51.4 | NC_004156.1 | + | 39917 | 1.05 | 0.015753 |
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Target: 5'- cACUACUAGUGGUAGUACUGCUGCCAUu -3' miRNA: 3'- -UGAUGAUCACCAUCAUGACGACGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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