Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16632 | 5' | -60.8 | NC_004156.1 | + | 91771 | 0.66 | 0.759118 |
Target: 5'- uGGCCucGGAcUCGGAGUCgguGGCGUCCAGGa -3' miRNA: 3'- -UCGG--UCUcGGUCUCGG---UCGCGGGUUC- -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 151508 | 0.66 | 0.749904 |
Target: 5'- -aCC-GAGuCCGGgaaAGCUAGCGUCCAAGa -3' miRNA: 3'- ucGGuCUC-GGUC---UCGGUCGCGGGUUC- -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 148758 | 0.66 | 0.740594 |
Target: 5'- uAGCCc--GCCAGAGCCu-UGCCCAu- -3' miRNA: 3'- -UCGGucuCGGUCUCGGucGCGGGUuc -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 99463 | 0.67 | 0.721722 |
Target: 5'- cAGCCAaguacggaaucGAcGUCGGAGCCGGUGUCguGGa -3' miRNA: 3'- -UCGGU-----------CU-CGGUCUCGGUCGCGGguUC- -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 80949 | 0.67 | 0.712175 |
Target: 5'- uGGCCGGcGUUAGAGCCc-UGCCCAc- -3' miRNA: 3'- -UCGGUCuCGGUCUCGGucGCGGGUuc -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 224350 | 0.67 | 0.6929 |
Target: 5'- cAGCCAcagcaaaccGAGCCAauggcaaccGAGCCAGUGguaaCCGAGc -3' miRNA: 3'- -UCGGU---------CUCGGU---------CUCGGUCGCg---GGUUC- -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 69660 | 0.68 | 0.644031 |
Target: 5'- cGCgCAGAGCCGGAGgCAGaCaCUCGGGa -3' miRNA: 3'- uCG-GUCUCGGUCUCgGUC-GcGGGUUC- -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 149221 | 0.68 | 0.634202 |
Target: 5'- aAGCC---GCUAGAGguugCAGCGCCCGAGu -3' miRNA: 3'- -UCGGucuCGGUCUCg---GUCGCGGGUUC- -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 58773 | 0.68 | 0.624371 |
Target: 5'- -aCCGGAGCCAGAGCUaccaguaccagAGCuaCCAGu -3' miRNA: 3'- ucGGUCUCGGUCUCGG-----------UCGcgGGUUc -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 73098 | 0.69 | 0.585168 |
Target: 5'- gAGUuuUAGAGUCAGAGUagcguuUAGCGUCCGAGu -3' miRNA: 3'- -UCG--GUCUCGGUCUCG------GUCGCGGGUUC- -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 23882 | 0.7 | 0.517974 |
Target: 5'- cGCCAGAGUCAGAGUCAcCGaguuaCCAGa -3' miRNA: 3'- uCGGUCUCGGUCUCGGUcGCg----GGUUc -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 64928 | 0.76 | 0.249026 |
Target: 5'- cGGCCAGAGCCAGAuaC--CGCCCGAGc -3' miRNA: 3'- -UCGGUCUCGGUCUcgGucGCGGGUUC- -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 43527 | 0.79 | 0.150736 |
Target: 5'- gAGCCAGAGCCcucuaaAGAGCCAGaGCCCu-- -3' miRNA: 3'- -UCGGUCUCGG------UCUCGGUCgCGGGuuc -5' |
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16632 | 5' | -60.8 | NC_004156.1 | + | 40788 | 1.07 | 0.001737 |
Target: 5'- gAGCCAGAGCCAGAGCCAGCGCCCAAGc -3' miRNA: 3'- -UCGGUCUCGGUCUCGGUCGCGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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