Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16634 | 3' | -54.1 | NC_004156.1 | + | 58511 | 0.7 | 0.823851 |
|
Target: 5'- aAACGUAAAGCcGAuGCUGAaGCAGCc -3' miRNA: 3'- -UUGCAUUUCGaCUcCGACUcCGUCGu -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 58544 | 0.7 | 0.856865 |
|
Target: 5'- aAGCGaAAGGCUGAuGCUGAaGCAGCc -3' miRNA: 3'- -UUGCaUUUCGACUcCGACUcCGUCGu -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 58571 | 0.74 | 0.617078 |
|
Target: 5'- aAACGUAAAGCcGAuGCUGAaGCAGCAa -3' miRNA: 3'- -UUGCAUUUCGaCUcCGACUcCGUCGU- -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 58625 | 0.74 | 0.617078 |
|
Target: 5'- aAACGUAAAGCcGAuGCUGAaGCAGCAa -3' miRNA: 3'- -UUGCAUUUCGaCUcCGACUcCGUCGU- -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 59105 | 0.66 | 0.956763 |
|
Target: 5'- gGACGaagAGAGUcguaagGAGGCUGcuGGUAGCAc -3' miRNA: 3'- -UUGCa--UUUCGa-----CUCCGACu-CCGUCGU- -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 85640 | 0.72 | 0.72 |
|
Target: 5'- aGACGUAAAGCggGAGGCuccuuUGAagcGGUAGCGu -3' miRNA: 3'- -UUGCAUUUCGa-CUCCG-----ACU---CCGUCGU- -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 224714 | 0.78 | 0.429635 |
|
Target: 5'- uGAgGuUGAGGUUGAGGUUGAGGCAGUu -3' miRNA: 3'- -UUgC-AUUUCGACUCCGACUCCGUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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