Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16634 | 3' | -54.1 | NC_004156.1 | + | 57966 | 0.86 | 0.142104 |
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Target: 5'- aAACGUAAAGCcGAGGCUGAaGCAGCAa -3' miRNA: 3'- -UUGCAUUUCGaCUCCGACUcCGUCGU- -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 58296 | 0.86 | 0.142104 |
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Target: 5'- aAACGUAAAGCcGAGGCUGAaGCAGCAa -3' miRNA: 3'- -UUGCAUUUCGaCUCCGACUcCGUCGU- -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 57642 | 0.87 | 0.134856 |
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Target: 5'- aAACGcAAAGCgGAGGCUGAGGCAGCc -3' miRNA: 3'- -UUGCaUUUCGaCUCCGACUCCGUCGu -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 58419 | 0.98 | 0.026284 |
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Target: 5'- aAACGUAAAGCgGAGGCUGAGGCGGCAg -3' miRNA: 3'- -UUGCAUUUCGaCUCCGACUCCGUCGU- -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 57762 | 1.03 | 0.012354 |
|
Target: 5'- aAACGUAAAGCUGAGGCUGAGGCAGCc -3' miRNA: 3'- -UUGCAUUUCGACUCCGACUCCGUCGu -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 57852 | 1.03 | 0.012354 |
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Target: 5'- aAACGUAAAGCUGAGGCUGAGGCAGCc -3' miRNA: 3'- -UUGCAUUUCGACUCCGACUCCGUCGu -5' |
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| 16634 | 3' | -54.1 | NC_004156.1 | + | 57909 | 1.07 | 0.006848 |
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Target: 5'- aAACGUAAAGCUGAGGCUGAGGCAGCAa -3' miRNA: 3'- -UUGCAUUUCGACUCCGACUCCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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