Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16635 | 5' | -56.9 | NC_004156.1 | + | 58173 | 0.66 | 0.860397 |
Target: 5'- aAAGCcGAGGCUGAAGCgGCaGCcAAAc -3' miRNA: 3'- -UUCGcCUCCGACUUCGaCGgCGaUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 58233 | 0.66 | 0.860397 |
Target: 5'- aAAGCcGAGGCUGAAGCgGCaGCcAAAc -3' miRNA: 3'- -UUCGcCUCCGACUUCGaCGgCGaUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 58290 | 0.67 | 0.819544 |
Target: 5'- aAAGCcGAGGCUGAAGCaGCaaaGCg--- -3' miRNA: 3'- -UUCGcCUCCGACUUCGaCGg--CGauuu -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 57756 | 0.68 | 0.792912 |
Target: 5'- aAAGCuGAGGCUGAGGCaGCCa----- -3' miRNA: 3'- -UUCGcCUCCGACUUCGaCGGcgauuu -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 57846 | 0.68 | 0.792912 |
Target: 5'- aAAGCuGAGGCUGAGGCaGCCa----- -3' miRNA: 3'- -UUCGcCUCCGACUUCGaCGGcgauuu -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 57960 | 0.68 | 0.78742 |
Target: 5'- aAAGCcGAGGCUGAAGCaGCaaaacgaaaggccgaUGCUGAAg -3' miRNA: 3'- -UUCGcCUCCGACUUCGaCG---------------GCGAUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 57903 | 0.68 | 0.768759 |
Target: 5'- aAAGCuGAGGCUGAGGCaGCaaaacgaaaggccgaUGCUGAAg -3' miRNA: 3'- -UUCGcCUCCGACUUCGaCG---------------GCGAUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 191477 | 0.7 | 0.69603 |
Target: 5'- --cCGGAGGUggGuAAGCUGUCGCUGGAc -3' miRNA: 3'- uucGCCUCCGa-C-UUCGACGGCGAUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 58413 | 0.77 | 0.315318 |
Target: 5'- aAAGCGGAGGCUGAGGCgGCaGCcAAAc -3' miRNA: 3'- -UUCGCCUCCGACUUCGaCGgCGaUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 57636 | 0.82 | 0.160717 |
Target: 5'- aAAGCGGAGGCUGAGGCaGCCaaacgcaaagccgauGCUGAAg -3' miRNA: 3'- -UUCGCCUCCGACUUCGaCGG---------------CGAUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 58113 | 0.82 | 0.160717 |
Target: 5'- aAAGCGGAGGCUGAAGCaGCCgaagccaaacguaaaGCUGAGa -3' miRNA: 3'- -UUCGCCUCCGACUUCGaCGG---------------CGAUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 58383 | 0.82 | 0.159081 |
Target: 5'- aAAGCGGAGGCUGAAGCaGCCGaacgAAAa -3' miRNA: 3'- -UUCGCCUCCGACUUCGaCGGCga--UUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 57729 | 0.86 | 0.084804 |
Target: 5'- aAAGCGGAGGCUGAAGCUGCUGaacgAAAa -3' miRNA: 3'- -UUCGCCUCCGACUUCGACGGCga--UUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 57819 | 0.86 | 0.084804 |
Target: 5'- aAAGCGGAGGCUGAAGCUGCUGaacgAAAa -3' miRNA: 3'- -UUCGCCUCCGACUUCGACGGCga--UUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 58263 | 0.91 | 0.036588 |
Target: 5'- aAAGCGGAGGCUGAAGCUGCUGCcAAAc -3' miRNA: 3'- -UUCGCCUCCGACUUCGACGGCGaUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 57666 | 0.92 | 0.031895 |
Target: 5'- aAAGCcGAGGCUGAAGCUGCCGCUAAAc -3' miRNA: 3'- -UUCGcCUCCGACUUCGACGGCGAUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 58050 | 0.95 | 0.019958 |
Target: 5'- aAAGCGGAGGCUGAAGCUGCCGCcAAAc -3' miRNA: 3'- -UUCGCCUCCGACUUCGACGGCGaUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 58203 | 0.95 | 0.019958 |
Target: 5'- aAAGCGGAGGCUGAAGCUGCCGCcAAAc -3' miRNA: 3'- -UUCGCCUCCGACUUCGACGGCGaUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 57990 | 1.03 | 0.005718 |
Target: 5'- aAAGCGGAGGCUGAAGCUGCCGCUAAAc -3' miRNA: 3'- -UUCGCCUCCGACUUCGACGGCGAUUU- -5' |
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16635 | 5' | -56.9 | NC_004156.1 | + | 58020 | 1.03 | 0.005718 |
Target: 5'- aAAGCGGAGGCUGAAGCUGCCGCUAAAc -3' miRNA: 3'- -UUCGCCUCCGACUUCGACGGCGAUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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