miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16636 3' -47.3 NC_004156.1 + 91999 0.66 0.999979
Target:  5'- cAAGC-GUAggcGUCAGGUUGACGAUg- -3'
miRNA:   3'- uUUCGaUAUa--CAGUUCAGCUGCUGgg -5'
16636 3' -47.3 NC_004156.1 + 184846 0.67 0.99992
Target:  5'- -uGGCUA-AUGUCGAGUCGGCcaaacuuugaaauuGCCUa -3'
miRNA:   3'- uuUCGAUaUACAGUUCAGCUGc-------------UGGG- -5'
16636 3' -47.3 NC_004156.1 + 75372 0.68 0.999807
Target:  5'- gAAGGCgAUAuUGcCAAGUUGACGGCUUu -3'
miRNA:   3'- -UUUCGaUAU-ACaGUUCAGCUGCUGGG- -5'
16636 3' -47.3 NC_004156.1 + 9727 0.69 0.998628
Target:  5'- cAAAGUgAUGUGgaUCAGGUCGACGAaCUg -3'
miRNA:   3'- -UUUCGaUAUAC--AGUUCAGCUGCUgGG- -5'
16636 3' -47.3 NC_004156.1 + 75602 0.69 0.999074
Target:  5'- -cAGuCUGUGUGUCAGG-CuGCGGCCa -3'
miRNA:   3'- uuUC-GAUAUACAGUUCaGcUGCUGGg -5'
16636 3' -47.3 NC_004156.1 + 62913 1.12 0.019468
Target:  5'- cAAAGCUAUAUGUCAAGUCGACGACCCg -3'
miRNA:   3'- -UUUCGAUAUACAGUUCAGCUGCUGGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.