Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16639 | 5' | -47.4 | NC_004156.1 | + | 147889 | 0.66 | 0.999943 |
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Target: 5'- cUUGGcUAgcacCUCCAAUUgaucGCGCUUCAc -3' miRNA: 3'- aGACCuAU----GAGGUUAAa---CGCGAAGUa -5' |
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| 16639 | 5' | -47.4 | NC_004156.1 | + | 52262 | 0.67 | 0.99987 |
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Target: 5'- -aUGGGUGCUUCAAagagcgagucUUUGCGUUgcgCAUa -3' miRNA: 3'- agACCUAUGAGGUU----------AAACGCGAa--GUA- -5' |
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| 16639 | 5' | -47.4 | NC_004156.1 | + | 148432 | 0.67 | 0.999724 |
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Target: 5'- uUUUGGGUACUCUuAUUUGgGUUUUg- -3' miRNA: 3'- -AGACCUAUGAGGuUAAACgCGAAGua -5' |
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| 16639 | 5' | -47.4 | NC_004156.1 | + | 35193 | 0.68 | 0.999449 |
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Target: 5'- cCUG-AUGCUCCAccAUUUGUGCUgCAa -3' miRNA: 3'- aGACcUAUGAGGU--UAAACGCGAaGUa -5' |
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| 16639 | 5' | -47.4 | NC_004156.1 | + | 114529 | 0.69 | 0.998466 |
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Target: 5'- aCUGGAUugUCCAAaugaucguacUUgacugcggaaaUGUGCUUCAc -3' miRNA: 3'- aGACCUAugAGGUU----------AA-----------ACGCGAAGUa -5' |
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| 16639 | 5' | -47.4 | NC_004156.1 | + | 89793 | 0.69 | 0.99815 |
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Target: 5'- gUUUGGAUagGCUCUGacgcacGUUUGCGUUUCAc -3' miRNA: 3'- -AGACCUA--UGAGGU------UAAACGCGAAGUa -5' |
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| 16639 | 5' | -47.4 | NC_004156.1 | + | 61911 | 0.69 | 0.99815 |
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Target: 5'- -gUGGAUAaUCCGGUUaGCGUUUCGg -3' miRNA: 3'- agACCUAUgAGGUUAAaCGCGAAGUa -5' |
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| 16639 | 5' | -47.4 | NC_004156.1 | + | 42674 | 0.71 | 0.994051 |
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Target: 5'- gUUUGGAUGCUCCAAg--GUGCa---- -3' miRNA: 3'- -AGACCUAUGAGGUUaaaCGCGaagua -5' |
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| 16639 | 5' | -47.4 | NC_004156.1 | + | 79076 | 1.08 | 0.028936 |
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Target: 5'- cUCUGGAUACUCCAAUUUGCGCUUCAUg -3' miRNA: 3'- -AGACCUAUGAGGUUAAACGCGAAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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