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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16640 | 5' | -50.7 | NC_004156.1 | + | 89067 | 0.66 | 0.998126 |
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Target: 5'- --gAGUCUGUUGccaguaggucaccgaGUCCGGUaaaGAGUGCa -3' miRNA: 3'- acgUCAGACAGC---------------CAGGUCAa--CUUACG- -5' |
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| 16640 | 5' | -50.7 | NC_004156.1 | + | 213966 | 0.67 | 0.9961 |
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Target: 5'- aUGcCAGUgUacUGGUCCAGUUGGAcGCg -3' miRNA: 3'- -AC-GUCAgAcaGCCAGGUCAACUUaCG- -5' |
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| 16640 | 5' | -50.7 | NC_004156.1 | + | 14511 | 0.68 | 0.991912 |
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Target: 5'- --gGGUCcgUGUgcaUGGUCCAGUUGAcuuUGCg -3' miRNA: 3'- acgUCAG--ACA---GCCAGGUCAACUu--ACG- -5' |
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| 16640 | 5' | -50.7 | NC_004156.1 | + | 188429 | 0.7 | 0.967747 |
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Target: 5'- cGUAGUCUGaaUCGGUCuCAGUcUGGucaGCa -3' miRNA: 3'- aCGUCAGAC--AGCCAG-GUCA-ACUua-CG- -5' |
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| 16640 | 5' | -50.7 | NC_004156.1 | + | 94109 | 0.73 | 0.901118 |
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Target: 5'- cGCAauUCUGUugcUGGUUCAGUUGAAUGUu -3' miRNA: 3'- aCGUc-AGACA---GCCAGGUCAACUUACG- -5' |
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| 16640 | 5' | -50.7 | NC_004156.1 | + | 81272 | 1.14 | 0.006719 |
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Target: 5'- aUGCAGUCUGUCGGUCCAGUUGAAUGCa -3' miRNA: 3'- -ACGUCAGACAGCCAGGUCAACUUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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