Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16644 | 5' | -47.7 | NC_004156.1 | + | 175639 | 0.66 | 0.999991 |
Target: 5'- uGCGACugAUGagGCcaacgaggaGGACAC-UGACGa -3' miRNA: 3'- -CGUUGugUACa-UG---------CCUGUGaACUGCc -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 186294 | 0.66 | 0.999991 |
Target: 5'- cGCuauGCACA---GCGGGCAg-UGAUGGa -3' miRNA: 3'- -CGu--UGUGUacaUGCCUGUgaACUGCC- -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 99790 | 0.66 | 0.999988 |
Target: 5'- uCGACAC-UGUAUGGAgGCggUGACc- -3' miRNA: 3'- cGUUGUGuACAUGCCUgUGa-ACUGcc -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 191566 | 0.66 | 0.999969 |
Target: 5'- uCGACACGUGUACcGACAacuCUUGGaacgaGGc -3' miRNA: 3'- cGUUGUGUACAUGcCUGU---GAACUg----CC- -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 202755 | 0.67 | 0.999958 |
Target: 5'- gGUAGCAC-UGUugAUGGgaGCACUauUGAUGGg -3' miRNA: 3'- -CGUUGUGuACA--UGCC--UGUGA--ACUGCC- -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 16517 | 0.67 | 0.999945 |
Target: 5'- cCGGuCACGUaauCGGACACUUGaaGCGGu -3' miRNA: 3'- cGUU-GUGUAcauGCCUGUGAAC--UGCC- -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 212370 | 0.7 | 0.998357 |
Target: 5'- cGCAcACACAUGUAUGuGCACaUGAauCGGu -3' miRNA: 3'- -CGU-UGUGUACAUGCcUGUGaACU--GCC- -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 83969 | 0.7 | 0.998035 |
Target: 5'- cGCuuCACGUGcgGCGGugGCUcGAUGa -3' miRNA: 3'- -CGuuGUGUACa-UGCCugUGAaCUGCc -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 131645 | 0.7 | 0.998035 |
Target: 5'- -gGACgACAUGgACGGAgACgggGACGGa -3' miRNA: 3'- cgUUG-UGUACaUGCCUgUGaa-CUGCC- -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 93234 | 0.72 | 0.994312 |
Target: 5'- uGUAAaagaGCAUGUACGGAaacagguugacguugUACUUGGCGu -3' miRNA: 3'- -CGUUg---UGUACAUGCCU---------------GUGAACUGCc -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 150460 | 0.72 | 0.990856 |
Target: 5'- gGC-ACGCGUGguggucgGCGGACACUUGugcugcacauCGGu -3' miRNA: 3'- -CGuUGUGUACa------UGCCUGUGAACu---------GCC- -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 103948 | 0.74 | 0.976308 |
Target: 5'- uGUAAUACAUGUugGGACAaagcacACGGu -3' miRNA: 3'- -CGUUGUGUACAugCCUGUgaac--UGCC- -5' |
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16644 | 5' | -47.7 | NC_004156.1 | + | 101880 | 1.13 | 0.018037 |
Target: 5'- uGCAACACAUGUACGGACACUUGACGGc -3' miRNA: 3'- -CGUUGUGUACAUGCCUGUGAACUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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