Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16645 | 3' | -51.3 | NC_004156.1 | + | 149270 | 0.66 | 0.99779 |
Target: 5'- uGUAGaaGGUGguGUCGGUGUACgAGGAg -3' miRNA: 3'- -CGUUggCCAU--CAGCCACGUGaUCUUg -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 136460 | 0.66 | 0.99779 |
Target: 5'- gGCAAagaCGGUAcGUCuGGUG-ACUAGGAa -3' miRNA: 3'- -CGUUg--GCCAU-CAG-CCACgUGAUCUUg -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 125931 | 0.66 | 0.997383 |
Target: 5'- cGCAGagUGGUAGUUGaugacgGCACUGGGAUc -3' miRNA: 3'- -CGUUg-GCCAUCAGCca----CGUGAUCUUG- -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 205616 | 0.66 | 0.996383 |
Target: 5'- uGCGAUCGGUAcaCgGGUGCACagaUGGAGg -3' miRNA: 3'- -CGUUGGCCAUcaG-CCACGUG---AUCUUg -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 104323 | 0.67 | 0.995775 |
Target: 5'- uGCAACCGagGGaUCGGUGCGCa----- -3' miRNA: 3'- -CGUUGGCcaUC-AGCCACGUGaucuug -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 44703 | 0.67 | 0.995775 |
Target: 5'- aGCAgauGCCGGUGcuugaGGUGCugUgcacAGAGCu -3' miRNA: 3'- -CGU---UGGCCAUcag--CCACGugA----UCUUG- -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 134386 | 0.67 | 0.995775 |
Target: 5'- uGCAAgaguaUGGU-GUCGGUGCACUu--GCg -3' miRNA: 3'- -CGUUg----GCCAuCAGCCACGUGAucuUG- -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 206054 | 0.67 | 0.993438 |
Target: 5'- uGCAucggagaguACCGGcacUGGUacCGGUGCugucACUGGAGCc -3' miRNA: 3'- -CGU---------UGGCC---AUCA--GCCACG----UGAUCUUG- -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 136552 | 0.67 | 0.993438 |
Target: 5'- -aGACCGGgugacgAGUCGGgGCACauUAGAc- -3' miRNA: 3'- cgUUGGCCa-----UCAGCCaCGUG--AUCUug -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 44708 | 0.68 | 0.991372 |
Target: 5'- gGCGACCGGUGccGUaccaGGcGC-CUAGGAUg -3' miRNA: 3'- -CGUUGGCCAU--CAg---CCaCGuGAUCUUG- -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 213954 | 0.68 | 0.991372 |
Target: 5'- gGCAGCgaagAGUCGGUGUugUacaAGAGCg -3' miRNA: 3'- -CGUUGgccaUCAGCCACGugA---UCUUG- -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 286 | 0.68 | 0.987346 |
Target: 5'- aGCAACCGGUAGaguagcaccaCaGUGCAgugUAGAACa -3' miRNA: 3'- -CGUUGGCCAUCa---------GcCACGUg--AUCUUG- -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 169659 | 0.69 | 0.97513 |
Target: 5'- cCAGuCCaGGUAGUCGGUGUAUUuaAACa -3' miRNA: 3'- cGUU-GG-CCAUCAGCCACGUGAucUUG- -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 191231 | 0.7 | 0.966509 |
Target: 5'- cGCaAACUGGUGGUCG-UGCACgauGAGg -3' miRNA: 3'- -CG-UUGGCCAUCAGCcACGUGau-CUUg -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 184121 | 0.74 | 0.840312 |
Target: 5'- gGCGAUCGGUAGU--GUGCugUGGAAg -3' miRNA: 3'- -CGUUGGCCAUCAgcCACGugAUCUUg -5' |
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16645 | 3' | -51.3 | NC_004156.1 | + | 103310 | 1.13 | 0.006955 |
Target: 5'- uGCAACCGGUAGUCGGUGCACUAGAACg -3' miRNA: 3'- -CGUUGGCCAUCAGCCACGUGAUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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