Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16645 | 5' | -46.5 | NC_004156.1 | + | 53109 | 0.66 | 0.999995 |
Target: 5'- -gAUGCAAACGUacaacGCCAAcuaUAgCGGUGa -3' miRNA: 3'- caUACGUUUGUA-----UGGUUa--GUgGCCAC- -5' |
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16645 | 5' | -46.5 | NC_004156.1 | + | 182020 | 0.66 | 0.999996 |
Target: 5'- -aGUGCAAAaacCGUacugGCCAGUCGgccacauuCCGGUGu -3' miRNA: 3'- caUACGUUU---GUA----UGGUUAGU--------GGCCAC- -5' |
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16645 | 5' | -46.5 | NC_004156.1 | + | 201961 | 0.66 | 0.999996 |
Target: 5'- -cGUGCAAGaccACCGA-CACCGGa- -3' miRNA: 3'- caUACGUUUguaUGGUUaGUGGCCac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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