Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16648 | 5' | -50.8 | NC_004156.1 | + | 129601 | 0.65 | 0.998071 |
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Target: 5'- -cGAACGUCaauugggcgaagagUUUGaGUGCGU-CAGCUu -3' miRNA: 3'- aaCUUGCAG--------------AAAC-CACGCAuGUCGGu -5' |
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| 16648 | 5' | -50.8 | NC_004156.1 | + | 143625 | 0.66 | 0.997406 |
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Target: 5'- aUGGAC-UCUUgcaccaGGaUGCGUuuGCAGCCAu -3' miRNA: 3'- aACUUGcAGAAa-----CC-ACGCA--UGUCGGU- -5' |
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| 16648 | 5' | -50.8 | NC_004156.1 | + | 213880 | 0.67 | 0.995358 |
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Target: 5'- gUUGaAACGUCUcgacgccagaguacUGGUGCGUACcgugcaccgAGCCGa -3' miRNA: 3'- -AAC-UUGCAGAa-------------ACCACGCAUG---------UCGGU- -5' |
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| 16648 | 5' | -50.8 | NC_004156.1 | + | 163192 | 0.68 | 0.989996 |
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Target: 5'- gUGAGCGUCgccaaUGGUGUGUACGaacucgugcGCUu -3' miRNA: 3'- aACUUGCAGaa---ACCACGCAUGU---------CGGu -5' |
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| 16648 | 5' | -50.8 | NC_004156.1 | + | 213652 | 0.71 | 0.950218 |
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Target: 5'- cUGGAaggGUCUggUGGUGCGcACAGUCAc -3' miRNA: 3'- aACUUg--CAGAa-ACCACGCaUGUCGGU- -5' |
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| 16648 | 5' | -50.8 | NC_004156.1 | + | 39265 | 0.72 | 0.925789 |
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Target: 5'- aUGGGCGUCUUUGGUGgagACAuGCUAg -3' miRNA: 3'- aACUUGCAGAAACCACgcaUGU-CGGU- -5' |
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| 16648 | 5' | -50.8 | NC_004156.1 | + | 112185 | 1.06 | 0.01644 |
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Target: 5'- gUUGAACGUCUUUGGUGCGUACAGCCAc -3' miRNA: 3'- -AACUUGCAGAAACCACGCAUGUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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