miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16649 3' -55.8 NC_004156.1 + 22584 0.7 0.806391
Target:  5'- uCGGUGCucgGAGC-UCGGUGCuCGGUGCUu -3'
miRNA:   3'- -GCCACG---UUCGuGGUCACG-GUCAUGGu -5'
16649 3' -55.8 NC_004156.1 + 68228 0.7 0.806391
Target:  5'- --cUGCGAGuCugCAGUGCUGGUACUg -3'
miRNA:   3'- gccACGUUC-GugGUCACGGUCAUGGu -5'
16649 3' -55.8 NC_004156.1 + 49817 0.73 0.622778
Target:  5'- ---aGC-AGUAUCAGUGCUAGUACCAg -3'
miRNA:   3'- gccaCGuUCGUGGUCACGGUCAUGGU- -5'
16649 3' -55.8 NC_004156.1 + 206314 0.76 0.485207
Target:  5'- ---aGCAccGGUACCAGUGCCGGUACUc -3'
miRNA:   3'- gccaCGU--UCGUGGUCACGGUCAUGGu -5'
16649 3' -55.8 NC_004156.1 + 68513 0.77 0.439473
Target:  5'- aCGGUGCAucaaagAGCACUcagacuAGUGCCAgcaGUACCAg -3'
miRNA:   3'- -GCCACGU------UCGUGG------UCACGGU---CAUGGU- -5'
16649 3' -55.8 NC_004156.1 + 185930 0.88 0.099222
Target:  5'- uGGUGCuagugcuaguaccAGUACCAGUGCCAGUGCCAg -3'
miRNA:   3'- gCCACGu------------UCGUGGUCACGGUCAUGGU- -5'
16649 3' -55.8 NC_004156.1 + 123056 1.09 0.004028
Target:  5'- cCGGUGCAAGCACCAGUGCCAGUACCAg -3'
miRNA:   3'- -GCCACGUUCGUGGUCACGGUCAUGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.