Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16651 | 5' | -56.6 | NC_004156.1 | + | 196352 | 0.67 | 0.930562 |
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Target: 5'- -uGCugaaGAGgaagAGGCGGAGUCugAGCCGGc -3' miRNA: 3'- gcCGug--CUCa---UCUGCCUCAG--UCGGCU- -5' |
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| 16651 | 5' | -56.6 | NC_004156.1 | + | 149423 | 0.67 | 0.925491 |
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Target: 5'- uGGUA-GAGUAcGAUGGAG-CuGCCGAu -3' miRNA: 3'- gCCGUgCUCAU-CUGCCUCaGuCGGCU- -5' |
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| 16651 | 5' | -56.6 | NC_004156.1 | + | 92107 | 0.67 | 0.908951 |
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Target: 5'- gGGCAUcaaGGUGGACGGGGagCAGCUc- -3' miRNA: 3'- gCCGUGc--UCAUCUGCCUCa-GUCGGcu -5' |
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| 16651 | 5' | -56.6 | NC_004156.1 | + | 136228 | 0.68 | 0.890448 |
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Target: 5'- uGGCcCGAGUGcACGGAGUCua-CGAa -3' miRNA: 3'- gCCGuGCUCAUcUGCCUCAGucgGCU- -5' |
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| 16651 | 5' | -56.6 | NC_004156.1 | + | 146713 | 0.68 | 0.869343 |
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Target: 5'- cCGGCAagauguacgcucuCGAGU--GCGGAGUCgaAGCCGu -3' miRNA: 3'- -GCCGU-------------GCUCAucUGCCUCAG--UCGGCu -5' |
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| 16651 | 5' | -56.6 | NC_004156.1 | + | 143919 | 1.09 | 0.004662 |
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Target: 5'- uCGGCACGAGUAGACGGAGUCAGCCGAu -3' miRNA: 3'- -GCCGUGCUCAUCUGCCUCAGUCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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