Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16652 | 3' | -51.9 | NC_004156.1 | + | 61474 | 0.66 | 0.995801 |
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Target: 5'- aCAUUGCCCACAguACGugaccgucaGGUgCCAGUu-- -3' miRNA: 3'- -GUAACGGGUGU--UGC---------CCAaGGUCAugc -5' |
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| 16652 | 3' | -51.9 | NC_004156.1 | + | 175317 | 0.66 | 0.995801 |
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Target: 5'- cCGUUGCCCACAAagaaGaGGUUcucaaacauuaCCAGUuuGCa -3' miRNA: 3'- -GUAACGGGUGUUg---C-CCAA-----------GGUCA--UGc -5' |
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| 16652 | 3' | -51.9 | NC_004156.1 | + | 164368 | 0.66 | 0.994973 |
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Target: 5'- -cUUGUCgGCGAUGGGggugcugaaugaUCCAGUGCc -3' miRNA: 3'- guAACGGgUGUUGCCCa-----------AGGUCAUGc -5' |
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| 16652 | 3' | -51.9 | NC_004156.1 | + | 152330 | 0.73 | 0.880302 |
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Target: 5'- uGUUGCCCACAuCGGGcagcaCCAgGUACa -3' miRNA: 3'- gUAACGGGUGUuGCCCaa---GGU-CAUGc -5' |
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| 16652 | 3' | -51.9 | NC_004156.1 | + | 25381 | 0.73 | 0.857323 |
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Target: 5'- cCGUUGCCgAUAcuacagugccaaaACGGGUaCCAGUAUGa -3' miRNA: 3'- -GUAACGGgUGU-------------UGCCCAaGGUCAUGC- -5' |
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| 16652 | 3' | -51.9 | NC_004156.1 | + | 146461 | 1.1 | 0.009246 |
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Target: 5'- aCAUUGCCCACAACGGGUUCCAGUACGa -3' miRNA: 3'- -GUAACGGGUGUUGCCCAAGGUCAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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