Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
16655 | 3' | -54.2 | NC_004156.1 | + | 44570 | 0.68 | 0.934623 |
Target: 5'- gGCcUCUGGUGCagguaGGCuaGAGUGUUGCAa -3' miRNA: 3'- -CGcAGACCGUGgg---CCG--UUCAUAACGU- -5' |
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16655 | 3' | -54.2 | NC_004156.1 | + | 26139 | 0.72 | 0.78289 |
Target: 5'- aGCGUCUGGUACuaGGUGAGUG--GCu -3' miRNA: 3'- -CGCAGACCGUGggCCGUUCAUaaCGu -5' |
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16655 | 3' | -54.2 | NC_004156.1 | + | 83737 | 0.73 | 0.735624 |
Target: 5'- uGCGUUUGGCACaCUGGaCGAGacgUGCGg -3' miRNA: 3'- -CGCAGACCGUG-GGCC-GUUCauaACGU- -5' |
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16655 | 3' | -54.2 | NC_004156.1 | + | 180971 | 1.11 | 0.004619 |
Target: 5'- aGCGUCUGGCACCCGGCAAGUAUUGCAu -3' miRNA: 3'- -CGCAGACCGUGGGCCGUUCAUAACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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