Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16655 | 5' | -55.2 | NC_004156.1 | + | 214352 | 0.66 | 0.969488 |
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Target: 5'- aAG-GCAAUCa--GCACCGGCacaGUGg -3' miRNA: 3'- -UCaCGUUAGgagCGUGGCCGaugCAC- -5' |
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| 16655 | 5' | -55.2 | NC_004156.1 | + | 173030 | 0.66 | 0.959824 |
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Target: 5'- cGGUGCGAUCCUuucuaguguagUGCAUgaugGGCU-CGUGa -3' miRNA: 3'- -UCACGUUAGGA-----------GCGUGg---CCGAuGCAC- -5' |
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| 16655 | 5' | -55.2 | NC_004156.1 | + | 210491 | 0.66 | 0.95618 |
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Target: 5'- -cUGgAGcCCcUGCugCGGCUGCGUGa -3' miRNA: 3'- ucACgUUaGGaGCGugGCCGAUGCAC- -5' |
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| 16655 | 5' | -55.2 | NC_004156.1 | + | 140380 | 0.67 | 0.952318 |
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Target: 5'- gGGUGCA----UCG-GCCGGCUugGUGu -3' miRNA: 3'- -UCACGUuaggAGCgUGGCCGAugCAC- -5' |
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| 16655 | 5' | -55.2 | NC_004156.1 | + | 23438 | 0.68 | 0.901175 |
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Target: 5'- -aUGCGAUcgauugCCUCGCAguaaUCGGCUACGa- -3' miRNA: 3'- ucACGUUA------GGAGCGU----GGCCGAUGCac -5' |
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| 16655 | 5' | -55.2 | NC_004156.1 | + | 173936 | 0.69 | 0.888206 |
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Target: 5'- cGGUGCAcuUCCUCgGCGuuGGCgUACGa- -3' miRNA: 3'- -UCACGUu-AGGAG-CGUggCCG-AUGCac -5' |
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| 16655 | 5' | -55.2 | NC_004156.1 | + | 132691 | 0.69 | 0.888206 |
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Target: 5'- cAGUGCA---CUCGUACCGuuUACGUGc -3' miRNA: 3'- -UCACGUuagGAGCGUGGCcgAUGCAC- -5' |
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| 16655 | 5' | -55.2 | NC_004156.1 | + | 106260 | 0.69 | 0.85968 |
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Target: 5'- -aUGC-AUUCUCGCACCGGUagaGUGg -3' miRNA: 3'- ucACGuUAGGAGCGUGGCCGaugCAC- -5' |
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| 16655 | 5' | -55.2 | NC_004156.1 | + | 99738 | 0.7 | 0.836181 |
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Target: 5'- uAGUcGCGGUCCaCGaCACCGGCUccgACGUc -3' miRNA: 3'- -UCA-CGUUAGGaGC-GUGGCCGA---UGCAc -5' |
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| 16655 | 5' | -55.2 | NC_004156.1 | + | 180927 | 1.09 | 0.004879 |
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Target: 5'- aAGUGCAAUCCUCGCACCGGCUACGUGg -3' miRNA: 3'- -UCACGUUAGGAGCGUGGCCGAUGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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