Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16657 | 5' | -52.9 | NC_004156.1 | + | 92252 | 0.66 | 0.990056 |
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Target: 5'- aGCUGaUGGcUCGAAagguUGCCGAGGUGg- -3' miRNA: 3'- cCGAC-GCCaGGCUU----ACGGCUUCAUaa -5' |
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| 16657 | 5' | -52.9 | NC_004156.1 | + | 34112 | 0.66 | 0.985606 |
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Target: 5'- uGGCUGCuuguuccuGGUCCGggUGUguaAAGUAg- -3' miRNA: 3'- -CCGACG--------CCAGGCuuACGgc-UUCAUaa -5' |
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| 16657 | 5' | -52.9 | NC_004156.1 | + | 143667 | 0.67 | 0.977482 |
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Target: 5'- cGGCUGac-UCCGucuacucGUGCCGAAGUGg- -3' miRNA: 3'- -CCGACgccAGGCu------UACGGCUUCAUaa -5' |
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| 16657 | 5' | -52.9 | NC_004156.1 | + | 2454 | 0.68 | 0.97234 |
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Target: 5'- cGCUGCuGUCCGAAU-CCGAcucuGGUGc- -3' miRNA: 3'- cCGACGcCAGGCUUAcGGCU----UCAUaa -5' |
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| 16657 | 5' | -52.9 | NC_004156.1 | + | 135910 | 0.68 | 0.969473 |
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Target: 5'- uGGCUGUGcaCCGAGUaucgGUCGAAGUGc- -3' miRNA: 3'- -CCGACGCcaGGCUUA----CGGCUUCAUaa -5' |
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| 16657 | 5' | -52.9 | NC_004156.1 | + | 136238 | 0.69 | 0.951935 |
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Target: 5'- aGCUGCcaacuGGcCCGAGUGCaCGGAGUc-- -3' miRNA: 3'- cCGACG-----CCaGGCUUACG-GCUUCAuaa -5' |
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| 16657 | 5' | -52.9 | NC_004156.1 | + | 207438 | 1.08 | 0.009688 |
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Target: 5'- cGGCUGCGGUCCGAAUGCCGAAGUAUUc -3' miRNA: 3'- -CCGACGCCAGGCUUACGGCUUCAUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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