Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16659 | 3' | -47.1 | NC_004156.1 | + | 165957 | 0.66 | 0.999986 |
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Target: 5'- cCUCCAUCAAuuucuuuauaguguaAUCCCUCUGuuugauuuuACGcuUGAGu -3' miRNA: 3'- -GAGGUAGUU---------------UGGGGAGAU---------UGUu-ACUC- -5' |
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| 16659 | 3' | -47.1 | NC_004156.1 | + | 196581 | 0.66 | 0.999969 |
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Target: 5'- -gCCGUUAGA-CUCUUUGACGGUGGGc -3' miRNA: 3'- gaGGUAGUUUgGGGAGAUUGUUACUC- -5' |
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| 16659 | 3' | -47.1 | NC_004156.1 | + | 93067 | 0.67 | 0.999959 |
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Target: 5'- aCUCCAUCGGGCgUCUUUAAgGuUGAa -3' miRNA: 3'- -GAGGUAGUUUGgGGAGAUUgUuACUc -5' |
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| 16659 | 3' | -47.1 | NC_004156.1 | + | 40506 | 0.67 | 0.999945 |
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Target: 5'- cCUCaacaaaAGACCCCUgUAcCAAUGAGa -3' miRNA: 3'- -GAGguag--UUUGGGGAgAUuGUUACUC- -5' |
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| 16659 | 3' | -47.1 | NC_004156.1 | + | 103513 | 0.7 | 0.998052 |
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Target: 5'- aUCCAaUGAACCCguucaauguggccgaUUCUGACAAUGAGc -3' miRNA: 3'- gAGGUaGUUUGGG---------------GAGAUUGUUACUC- -5' |
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| 16659 | 3' | -47.1 | NC_004156.1 | + | 104654 | 0.7 | 0.997869 |
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Target: 5'- gUCCAUUGAugcaacgGCUCCUCUAugGCGGUGAa -3' miRNA: 3'- gAGGUAGUU-------UGGGGAGAU--UGUUACUc -5' |
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| 16659 | 3' | -47.1 | NC_004156.1 | + | 65293 | 0.71 | 0.997038 |
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Target: 5'- -gCCAUCGAACCCagu--ACGGUGAGu -3' miRNA: 3'- gaGGUAGUUUGGGgagauUGUUACUC- -5' |
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| 16659 | 3' | -47.1 | NC_004156.1 | + | 88604 | 0.71 | 0.996502 |
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Target: 5'- gCUCCA----ACCCUUCUAACAGUGc- -3' miRNA: 3'- -GAGGUaguuUGGGGAGAUUGUUACuc -5' |
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| 16659 | 3' | -47.1 | NC_004156.1 | + | 20562 | 0.78 | 0.886258 |
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Target: 5'- aUUCAUCGAAUCCCUCUAcGCAuAUGGGa -3' miRNA: 3'- gAGGUAGUUUGGGGAGAU-UGU-UACUC- -5' |
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| 16659 | 3' | -47.1 | NC_004156.1 | + | 217260 | 1.1 | 0.026991 |
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Target: 5'- cCUCCAUCAAACCCCUCUAACAAUGAGu -3' miRNA: 3'- -GAGGUAGUUUGGGGAGAUUGUUACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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