Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16659 | 5' | -50 | NC_004156.1 | + | 111308 | 0.66 | 0.999093 |
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Target: 5'- uGGCCGGCGaCAGAGcgugcaacagcgaaaACUGAGUcUGAc -3' miRNA: 3'- -UCGGUUGUaGUCUC---------------UGGCUCAaACUa -5' |
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| 16659 | 5' | -50 | NC_004156.1 | + | 193642 | 0.66 | 0.999018 |
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Target: 5'- uGCCAACGUCAaGGAgCGAGgagUGc- -3' miRNA: 3'- uCGGUUGUAGUcUCUgGCUCaa-ACua -5' |
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| 16659 | 5' | -50 | NC_004156.1 | + | 127243 | 0.66 | 0.999018 |
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Target: 5'- uGGUCAACuUUAGAGuguCCGAGgUUGGa -3' miRNA: 3'- -UCGGUUGuAGUCUCu--GGCUCaAACUa -5' |
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| 16659 | 5' | -50 | NC_004156.1 | + | 45352 | 0.66 | 0.998555 |
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Target: 5'- cAGCCAACAUCGGAGccACUGuGg----- -3' miRNA: 3'- -UCGGUUGUAGUCUC--UGGCuCaaacua -5' |
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| 16659 | 5' | -50 | NC_004156.1 | + | 213675 | 0.66 | 0.998555 |
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Target: 5'- aAGUCGGCAUCGGuaaAGugCGAGUa---- -3' miRNA: 3'- -UCGGUUGUAGUC---UCugGCUCAaacua -5' |
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| 16659 | 5' | -50 | NC_004156.1 | + | 83810 | 0.68 | 0.995254 |
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Target: 5'- aAGUCAGCAaggaUCAGAuGGCCGAGa-UGAa -3' miRNA: 3'- -UCGGUUGU----AGUCU-CUGGCUCaaACUa -5' |
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| 16659 | 5' | -50 | NC_004156.1 | + | 122638 | 0.68 | 0.993605 |
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Target: 5'- uAGUCGGCAUCGGGGACauGGUgaUUGGa -3' miRNA: 3'- -UCGGUUGUAGUCUCUGgcUCA--AACUa -5' |
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| 16659 | 5' | -50 | NC_004156.1 | + | 164869 | 0.69 | 0.988945 |
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Target: 5'- uGGCCAACGUguUGGAGuugcCCGuGUUUGAUc -3' miRNA: 3'- -UCGGUUGUA--GUCUCu---GGCuCAAACUA- -5' |
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| 16659 | 5' | -50 | NC_004156.1 | + | 23882 | 0.7 | 0.972169 |
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Target: 5'- cGCCAGaGUCAGAGucACCGAGUUa--- -3' miRNA: 3'- uCGGUUgUAGUCUC--UGGCUCAAacua -5' |
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| 16659 | 5' | -50 | NC_004156.1 | + | 217223 | 1.06 | 0.02215 |
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Target: 5'- aAGCCAACAUCAGAGACCGAGUUUGAUu -3' miRNA: 3'- -UCGGUUGUAGUCUCUGGCUCAAACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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