Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1666 | 3' | -56.8 | NC_001347.2 | + | 136110 | 0.67 | 0.903988 |
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Target: 5'- ----gGGCCUCGgCCGGGGAg-GGUg -3' miRNA: 3'- uuaagCCGGAGCaGGCCUCUgaCCAg -5' |
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| 1666 | 3' | -56.8 | NC_001347.2 | + | 184954 | 0.68 | 0.840546 |
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Target: 5'- --gUCGGCCggcgugggCGgcUCgCGGAGACUGGg- -3' miRNA: 3'- uuaAGCCGGa-------GC--AG-GCCUCUGACCag -5' |
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| 1666 | 3' | -56.8 | NC_001347.2 | + | 94334 | 0.69 | 0.807439 |
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Target: 5'- --cUCGGCgUCGU-CGGAGGCcGGUg -3' miRNA: 3'- uuaAGCCGgAGCAgGCCUCUGaCCAg -5' |
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| 1666 | 3' | -56.8 | NC_001347.2 | + | 100199 | 0.72 | 0.646225 |
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Target: 5'- ---gCGGCCUCGcccagguagCCGGAGACggcGGUUa -3' miRNA: 3'- uuaaGCCGGAGCa--------GGCCUCUGa--CCAG- -5' |
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| 1666 | 3' | -56.8 | NC_001347.2 | + | 58989 | 0.73 | 0.586511 |
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Target: 5'- cAGUUCGGCUUCGgacUCGGAGAgUGaGUCc -3' miRNA: 3'- -UUAAGCCGGAGCa--GGCCUCUgAC-CAG- -5' |
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| 1666 | 3' | -56.8 | NC_001347.2 | + | 113950 | 1.08 | 0.004342 |
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Target: 5'- cAAUUCGGCCUCGUCCGGAGACUGGUCg -3' miRNA: 3'- -UUAAGCCGGAGCAGGCCUCUGACCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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