Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16663 | 3' | -45.3 | NC_004156.1 | + | 214170 | 0.66 | 1 |
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Target: 5'- cAGAGUUgccGUCUCGGCucGgUGCAc-- -3' miRNA: 3'- -UCUCAAauuCAGAGCUG--UgACGUaau -5' |
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| 16663 | 3' | -45.3 | NC_004156.1 | + | 52580 | 0.66 | 0.999999 |
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Target: 5'- aAGAGUgaaGAGUUugUCGACugUGCc--- -3' miRNA: 3'- -UCUCAaa-UUCAG--AGCUGugACGuaau -5' |
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| 16663 | 3' | -45.3 | NC_004156.1 | + | 54194 | 0.66 | 0.999998 |
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Target: 5'- ------cAAGUCUCGAUGCUGUAg-- -3' miRNA: 3'- ucucaaaUUCAGAGCUGUGACGUaau -5' |
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| 16663 | 3' | -45.3 | NC_004156.1 | + | 3317 | 0.7 | 0.999709 |
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Target: 5'- cAGAGUUUGAGUUgUUGGCAUUGgGUg- -3' miRNA: 3'- -UCUCAAAUUCAG-AGCUGUGACgUAau -5' |
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| 16663 | 3' | -45.3 | NC_004156.1 | + | 25525 | 0.75 | 0.979271 |
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Target: 5'- -aAGUcUAAGUCUCGACACcUGCAc-- -3' miRNA: 3'- ucUCAaAUUCAGAGCUGUG-ACGUaau -5' |
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| 16663 | 3' | -45.3 | NC_004156.1 | + | 25354 | 1.08 | 0.048084 |
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Target: 5'- aAGAGUUUAAGUCUCGACACUGCAUUAc -3' miRNA: 3'- -UCUCAAAUUCAGAGCUGUGACGUAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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