Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16666 | 5' | -51.9 | NC_004156.1 | + | 172807 | 0.66 | 0.99721 |
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Target: 5'- cAGGCACCGUGGUCGgcaaucACUG-UGa-- -3' miRNA: 3'- cUCCGUGGCAUUAGCa-----UGACcACagc -5' |
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| 16666 | 5' | -51.9 | NC_004156.1 | + | 127837 | 0.66 | 0.99707 |
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Target: 5'- aGGGGUGacaacaaacugauuCCGUuccCGUACUcGGUGUCGg -3' miRNA: 3'- -CUCCGU--------------GGCAuuaGCAUGA-CCACAGC- -5' |
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| 16666 | 5' | -51.9 | NC_004156.1 | + | 152308 | 0.67 | 0.994018 |
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Target: 5'- cAGGUACaCGUAAUUGUGCU--UGUCu -3' miRNA: 3'- cUCCGUG-GCAUUAGCAUGAccACAGc -5' |
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| 16666 | 5' | -51.9 | NC_004156.1 | + | 102302 | 0.67 | 0.989753 |
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Target: 5'- -uGGCGgUGguGUUGuUACUGGUGUCGg -3' miRNA: 3'- cuCCGUgGCauUAGC-AUGACCACAGC- -5' |
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| 16666 | 5' | -51.9 | NC_004156.1 | + | 53594 | 0.68 | 0.985221 |
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Target: 5'- -uGGCACUgGUGGUgGUGCUGGUuUUGa -3' miRNA: 3'- cuCCGUGG-CAUUAgCAUGACCAcAGC- -5' |
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| 16666 | 5' | -51.9 | NC_004156.1 | + | 19746 | 0.69 | 0.974475 |
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Target: 5'- cGGGCAUCGcuGUCgGUAUcGGUGUCGu -3' miRNA: 3'- cUCCGUGGCauUAG-CAUGaCCACAGC- -5' |
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| 16666 | 5' | -51.9 | NC_004156.1 | + | 542 | 0.7 | 0.962462 |
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Target: 5'- uAGuGUACUGUAAUUGUaacaACUGGUGUUa -3' miRNA: 3'- cUC-CGUGGCAUUAGCA----UGACCACAGc -5' |
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| 16666 | 5' | -51.9 | NC_004156.1 | + | 53376 | 1.09 | 0.010992 |
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Target: 5'- cGAGGCACCGUAAUCGUACUGGUGUCGu -3' miRNA: 3'- -CUCCGUGGCAUUAGCAUGACCACAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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