Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16667 | 3' | -49.6 | NC_004156.1 | + | 135529 | 0.66 | 0.999644 |
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Target: 5'- uGGuuGaggGUAGUAGUgGU-GGUGUUg -3' miRNA: 3'- gCCggCaa-CAUCAUUAgCAuCCACAG- -5' |
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| 16667 | 3' | -49.6 | NC_004156.1 | + | 18147 | 0.66 | 0.99945 |
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Target: 5'- aGGuuGUgGUGGUAGUgGUAGGa--- -3' miRNA: 3'- gCCggCAaCAUCAUUAgCAUCCacag -5' |
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| 16667 | 3' | -49.6 | NC_004156.1 | + | 215530 | 0.66 | 0.999322 |
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Target: 5'- aCGGUgGaUGUAGUGuuuuugugcagaGUUGUAGG-GUCg -3' miRNA: 3'- -GCCGgCaACAUCAU------------UAGCAUCCaCAG- -5' |
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| 16667 | 3' | -49.6 | NC_004156.1 | + | 102295 | 0.67 | 0.999169 |
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Target: 5'- gCGGUgGUggcgGUGGU-GUUGUuacuGGUGUCg -3' miRNA: 3'- -GCCGgCAa---CAUCAuUAGCAu---CCACAG- -5' |
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| 16667 | 3' | -49.6 | NC_004156.1 | + | 160523 | 0.67 | 0.998227 |
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Target: 5'- aGGCUuaGUUGUAGgucUGAUUGUAGGUuuaGUUa -3' miRNA: 3'- gCCGG--CAACAUC---AUUAGCAUCCA---CAG- -5' |
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| 16667 | 3' | -49.6 | NC_004156.1 | + | 215476 | 0.68 | 0.995916 |
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Target: 5'- uGGUCGUcGauuaUGGUGGUCGU-GGUGUUg -3' miRNA: 3'- gCCGGCAaC----AUCAUUAGCAuCCACAG- -5' |
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| 16667 | 3' | -49.6 | NC_004156.1 | + | 73863 | 0.69 | 0.994475 |
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Target: 5'- uGGuuGUUGUGGUuguuGUUGUAGuUGUUg -3' miRNA: 3'- gCCggCAACAUCAu---UAGCAUCcACAG- -5' |
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| 16667 | 3' | -49.6 | NC_004156.1 | + | 72131 | 0.69 | 0.991572 |
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Target: 5'- gGGuuGUUGUAGUuauuGUUGUAGuUGUUg -3' miRNA: 3'- gCCggCAACAUCAu---UAGCAUCcACAG- -5' |
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| 16667 | 3' | -49.6 | NC_004156.1 | + | 124228 | 0.72 | 0.963423 |
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Target: 5'- aGGuuGUgUGUAG-GAUUGUAGGUGUa -3' miRNA: 3'- gCCggCA-ACAUCaUUAGCAUCCACAg -5' |
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| 16667 | 3' | -49.6 | NC_004156.1 | + | 54783 | 1.12 | 0.012955 |
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Target: 5'- gCGGCCGUUGUAGUAAUCGUAGGUGUCg -3' miRNA: 3'- -GCCGGCAACAUCAUUAGCAUCCACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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