Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16672 | 5' | -56.7 | NC_004156.1 | + | 26554 | 0.66 | 0.948697 |
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Target: 5'- gCUAUagACUAGacaacGCCAGaGACGGGACAg -3' miRNA: 3'- -GGUGg-UGAUC-----CGGUCaCUGCCCUGUg -5' |
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| 16672 | 5' | -56.7 | NC_004156.1 | + | 180668 | 0.68 | 0.890234 |
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Target: 5'- gCCACCACguaGCCGGUG-CGaGGauuGCACu -3' miRNA: 3'- -GGUGGUGaucCGGUCACuGC-CC---UGUG- -5' |
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| 16672 | 5' | -56.7 | NC_004156.1 | + | 81183 | 0.68 | 0.87677 |
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Target: 5'- cCgGCCACUG---CAGUGACuaGGGACACg -3' miRNA: 3'- -GgUGGUGAUccgGUCACUG--CCCUGUG- -5' |
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| 16672 | 5' | -56.7 | NC_004156.1 | + | 145970 | 0.72 | 0.695852 |
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Target: 5'- cCCAUCACUuuuGGCUAcagGACGGGuGCGCa -3' miRNA: 3'- -GGUGGUGAu--CCGGUca-CUGCCC-UGUG- -5' |
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| 16672 | 5' | -56.7 | NC_004156.1 | + | 93880 | 0.72 | 0.666456 |
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Target: 5'- gUCACCGgUAGGCCAauguUGACGauGGGCACc -3' miRNA: 3'- -GGUGGUgAUCCGGUc---ACUGC--CCUGUG- -5' |
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| 16672 | 5' | -56.7 | NC_004156.1 | + | 76779 | 1.11 | 0.002725 |
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Target: 5'- uCCACCACUAGGCCAGUGACGGGACACa -3' miRNA: 3'- -GGUGGUGAUCCGGUCACUGCCCUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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