miRNA: 3'- -UCAC----------CGGUUUGACaCGUAGCAGUuGC- -5'
miRNA: 3'- uCACCGGU--------------UUGACACGUAGCAGuuGC- -5'
miRNA: 3'- uCACcGGUUUGACaCGUaGCAGUUGC- -5'
miRNA: 3'- -UCACCGGUUUGACACGUAGCAGUUGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16674 | 5' | -50.6 | NC_004156.1 | + | 96175 | 0.71 | 0.956134 |
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Target: 5'- gAGUGcugaaacacuGCCAGAUUG-GCAUCGUCGuCGg -3' miRNA: 3'- -UCAC----------CGGUUUGACaCGUAGCAGUuGC- -5' |
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| 16674 | 5' | -50.6 | NC_004156.1 | + | 75349 | 0.73 | 0.896921 |
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Target: 5'- uGUGGCCGcagccugacacacaGACUGUGCAUUGUU--CGg -3' miRNA: 3'- uCACCGGU--------------UUGACACGUAGCAGuuGC- -5' |
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| 16674 | 5' | -50.6 | NC_004156.1 | + | 19495 | 0.76 | 0.767382 |
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Target: 5'- cGUGcCCAGACUGcGCAcCGUCAACGg -3' miRNA: 3'- uCACcGGUUUGACaCGUaGCAGUUGC- -5' |
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| 16674 | 5' | -50.6 | NC_004156.1 | + | 84505 | 1.11 | 0.010946 |
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Target: 5'- aAGUGGCCAAACUGUGCAUCGUCAACGg -3' miRNA: 3'- -UCACCGGUUUGACACGUAGCAGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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