Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16675 | 5' | -51.1 | NC_004156.1 | + | 88941 | 0.66 | 0.997006 |
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Target: 5'- cGCACUGCUcaaaCAAGUCAu--CGGCc- -3' miRNA: 3'- -CGUGACGAac--GUUCAGUuucGCCGug -5' |
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| 16675 | 5' | -51.1 | NC_004156.1 | + | 107177 | 0.66 | 0.996241 |
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Target: 5'- gGCACUGUguuuuugUUGCAAGcgauucugcacaguUCGAGGauGCGCu -3' miRNA: 3'- -CGUGACG-------AACGUUC--------------AGUUUCgcCGUG- -5' |
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| 16675 | 5' | -51.1 | NC_004156.1 | + | 116624 | 0.66 | 0.995802 |
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Target: 5'- gGCACaauuggGCUaccuguuguugauUGCAcGUCAAgacGGCGGCAa -3' miRNA: 3'- -CGUGa-----CGA-------------ACGUuCAGUU---UCGCCGUg -5' |
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| 16675 | 5' | -51.1 | NC_004156.1 | + | 68221 | 0.69 | 0.972046 |
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Target: 5'- -aGCUGCUcUGCGAGUCugcaguGCuGGUACu -3' miRNA: 3'- cgUGACGA-ACGUUCAGuuu---CG-CCGUG- -5' |
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| 16675 | 5' | -51.1 | NC_004156.1 | + | 45583 | 0.7 | 0.95888 |
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Target: 5'- -gGCUGCccagUGCAAGUCcauaGAGGUGGCc- -3' miRNA: 3'- cgUGACGa---ACGUUCAG----UUUCGCCGug -5' |
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| 16675 | 5' | -51.1 | NC_004156.1 | + | 104777 | 0.72 | 0.909181 |
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Target: 5'- cCACUGCgaGCAAGUCcguagugcaguuGGAG-GGCACg -3' miRNA: 3'- cGUGACGaaCGUUCAG------------UUUCgCCGUG- -5' |
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| 16675 | 5' | -51.1 | NC_004156.1 | + | 111833 | 0.72 | 0.882353 |
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Target: 5'- aCACUGCUacUGCAAGUgGuacugucuGCGGCAa -3' miRNA: 3'- cGUGACGA--ACGUUCAgUuu------CGCCGUg -5' |
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| 16675 | 5' | -51.1 | NC_004156.1 | + | 142841 | 0.73 | 0.859767 |
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Target: 5'- cGgACUGCgcaUGCAAG---AAGCGGCACu -3' miRNA: 3'- -CgUGACGa--ACGUUCaguUUCGCCGUG- -5' |
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| 16675 | 5' | -51.1 | NC_004156.1 | + | 90221 | 1.13 | 0.005799 |
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Target: 5'- uGCACUGCUUGCAAGUCAAAGCGGCACg -3' miRNA: 3'- -CGUGACGAACGUUCAGUUUCGCCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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