Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16676 | 5' | -55.3 | NC_004156.1 | + | 15378 | 0.66 | 0.969018 |
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Target: 5'- uGUAGUGG-AUGCCGuCACCaCCAucgGUAc -3' miRNA: 3'- -CGUCACCaUAUGGC-GUGG-GGUca-CGU- -5' |
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| 16676 | 5' | -55.3 | NC_004156.1 | + | 170959 | 0.66 | 0.965968 |
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Target: 5'- cCAGUGG-AUAUUGCAUCCUuguugugaAGUGUAg -3' miRNA: 3'- cGUCACCaUAUGGCGUGGGG--------UCACGU- -5' |
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| 16676 | 5' | -55.3 | NC_004156.1 | + | 152236 | 0.66 | 0.951664 |
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Target: 5'- aGCAGcGGUcgACUguugGCACCCaucguGGUGCGu -3' miRNA: 3'- -CGUCaCCAuaUGG----CGUGGGg----UCACGU- -5' |
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| 16676 | 5' | -55.3 | NC_004156.1 | + | 39183 | 0.68 | 0.912256 |
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Target: 5'- gGCGGUGGUGaAUCGauuCCUgGGUGCu -3' miRNA: 3'- -CGUCACCAUaUGGCgu-GGGgUCACGu -5' |
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| 16676 | 5' | -55.3 | NC_004156.1 | + | 45600 | 0.72 | 0.706757 |
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Target: 5'- aCAGUGGcuccgAUGuuGgCugCCCAGUGCAa -3' miRNA: 3'- cGUCACCa----UAUggC-GugGGGUCACGU- -5' |
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| 16676 | 5' | -55.3 | NC_004156.1 | + | 96156 | 1.11 | 0.00378 |
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Target: 5'- cGCAGUGGUAUACCGCACCCCAGUGCAc -3' miRNA: 3'- -CGUCACCAUAUGGCGUGGGGUCACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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