Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16677 | 5' | -52.4 | NC_004156.1 | + | 173020 | 0.66 | 0.988716 |
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Target: 5'- aGGugCACCacGGUGcgauccuuucuAGUGUAGUGCa -3' miRNA: 3'- aUCugGUGGcaCUAC-----------UCACAUCACGa -5' |
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| 16677 | 5' | -52.4 | NC_004156.1 | + | 193916 | 0.67 | 0.981812 |
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Target: 5'- -cGGCCAUCGUGAUGGuUGc-GUGCa -3' miRNA: 3'- auCUGGUGGCACUACUcACauCACGa -5' |
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| 16677 | 5' | -52.4 | NC_004156.1 | + | 171644 | 0.67 | 0.977352 |
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Target: 5'- aAGAuCCACCGcUGcUGGGcuUGUGGUGCc -3' miRNA: 3'- aUCU-GGUGGC-ACuACUC--ACAUCACGa -5' |
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| 16677 | 5' | -52.4 | NC_004156.1 | + | 49264 | 0.69 | 0.947144 |
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Target: 5'- aGGAUUACCGUGAugcUGAGUaGUAGUaGUa -3' miRNA: 3'- aUCUGGUGGCACU---ACUCA-CAUCA-CGa -5' |
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| 16677 | 5' | -52.4 | NC_004156.1 | + | 144870 | 0.7 | 0.932939 |
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Target: 5'- -uGACCGucuCCGUGAUGGGUGUGcauuuUGCa -3' miRNA: 3'- auCUGGU---GGCACUACUCACAUc----ACGa -5' |
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| 16677 | 5' | -52.4 | NC_004156.1 | + | 109062 | 1.06 | 0.012786 |
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Target: 5'- aUAGACCACCGUGAUGAGUGUAGUGCUc -3' miRNA: 3'- -AUCUGGUGGCACUACUCACAUCACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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