Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16680 | 3' | -56.6 | NC_004156.1 | + | 16194 | 0.66 | 0.93462 |
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Target: 5'- gACCGCCuc--CGGUGGgCGccucgguacgcAUGGGCa -3' miRNA: 3'- -UGGCGGuauuGCCACCgGC-----------UACUCGa -5' |
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| 16680 | 3' | -56.6 | NC_004156.1 | + | 58033 | 0.66 | 0.924485 |
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Target: 5'- uGCCGCCAaacgcaaAGCGGaGGCUGA--AGCUg -3' miRNA: 3'- -UGGCGGUa------UUGCCaCCGGCUacUCGA- -5' |
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| 16680 | 3' | -56.6 | NC_004156.1 | + | 164965 | 0.66 | 0.924485 |
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Target: 5'- uCUGCCGgaUGAUGGUGGCuCGcgGuGCc -3' miRNA: 3'- uGGCGGU--AUUGCCACCG-GCuaCuCGa -5' |
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| 16680 | 3' | -56.6 | NC_004156.1 | + | 54320 | 0.66 | 0.919077 |
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Target: 5'- cAUCGUCAaaguggaccUggUGGUGGCCGAUGGa-- -3' miRNA: 3'- -UGGCGGU---------AuuGCCACCGGCUACUcga -5' |
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| 16680 | 3' | -56.6 | NC_004156.1 | + | 58063 | 0.66 | 0.919077 |
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Target: 5'- aGCCGCCAaacguaaAGCGGaGGCUGA--AGCUg -3' miRNA: 3'- -UGGCGGUa------UUGCCaCCGGCUacUCGA- -5' |
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| 16680 | 3' | -56.6 | NC_004156.1 | + | 180913 | 0.68 | 0.867809 |
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Target: 5'- cACCGgCuacgUGGUGGCCGAcggucUGGGCUc -3' miRNA: 3'- -UGGCgGuauuGCCACCGGCU-----ACUCGA- -5' |
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| 16680 | 3' | -56.6 | NC_004156.1 | + | 102286 | 0.69 | 0.820827 |
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Target: 5'- gUCGCUGUGGCGGUGGUggCGGUGGuGUUg -3' miRNA: 3'- uGGCGGUAUUGCCACCG--GCUACU-CGA- -5' |
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| 16680 | 3' | -56.6 | NC_004156.1 | + | 83968 | 0.7 | 0.777138 |
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Target: 5'- -gCGCUucacGUGcgGCGGUGGCuCGAUGAGUUu -3' miRNA: 3'- ugGCGG----UAU--UGCCACCG-GCUACUCGA- -5' |
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| 16680 | 3' | -56.6 | NC_004156.1 | + | 75462 | 0.71 | 0.720734 |
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Target: 5'- aGCCGCCAagaaUGAUGGUGGCggUGGUGAcaaugGCa -3' miRNA: 3'- -UGGCGGU----AUUGCCACCG--GCUACU-----CGa -5' |
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| 16680 | 3' | -56.6 | NC_004156.1 | + | 113862 | 1.08 | 0.004496 |
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Target: 5'- cACCGCCAUAACGGUGGCCGAUGAGCUa -3' miRNA: 3'- -UGGCGGUAUUGCCACCGGCUACUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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