Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16680 | 5' | -51.1 | NC_004156.1 | + | 189893 | 0.66 | 0.996409 |
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Target: 5'- aCGAGaCUCcuUCGACG-CCCGGACAc-- -3' miRNA: 3'- -GUUC-GAGu-AGCUGUgGGGUCUGUaau -5' |
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| 16680 | 5' | -51.1 | NC_004156.1 | + | 180876 | 0.67 | 0.993041 |
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Target: 5'- gAGGCUCugcggcacacugCGACACCUUGGACAa-- -3' miRNA: 3'- gUUCGAGua----------GCUGUGGGGUCUGUaau -5' |
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| 16680 | 5' | -51.1 | NC_004156.1 | + | 163694 | 0.67 | 0.992432 |
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Target: 5'- aGAGCUCcUCGAugaCGCCCUuGACAa-- -3' miRNA: 3'- gUUCGAGuAGCU---GUGGGGuCUGUaau -5' |
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| 16680 | 5' | -51.1 | NC_004156.1 | + | 41420 | 0.69 | 0.971925 |
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Target: 5'- --uGCUCAUUGAUACCUUGGAUAa-- -3' miRNA: 3'- guuCGAGUAGCUGUGGGGUCUGUaau -5' |
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| 16680 | 5' | -51.1 | NC_004156.1 | + | 19173 | 0.7 | 0.958788 |
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Target: 5'- -uGGC-CAUCGACACCUCGGAa---- -3' miRNA: 3'- guUCGaGUAGCUGUGGGGUCUguaau -5' |
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| 16680 | 5' | -51.1 | NC_004156.1 | + | 145644 | 0.71 | 0.941004 |
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Target: 5'- uCAGGCgcucuuugacggCAUCGGCACUUCGGACAa-- -3' miRNA: 3'- -GUUCGa-----------GUAGCUGUGGGGUCUGUaau -5' |
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| 16680 | 5' | -51.1 | NC_004156.1 | + | 190037 | 0.79 | 0.557507 |
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Target: 5'- --cGCUCAUCGACACCCU-GGCAUg- -3' miRNA: 3'- guuCGAGUAGCUGUGGGGuCUGUAau -5' |
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| 16680 | 5' | -51.1 | NC_004156.1 | + | 157215 | 0.83 | 0.386694 |
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Target: 5'- gCAAGUUUAUCGGCACCCUGGGCAg-- -3' miRNA: 3'- -GUUCGAGUAGCUGUGGGGUCUGUaau -5' |
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| 16680 | 5' | -51.1 | NC_004156.1 | + | 113897 | 1.07 | 0.014319 |
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Target: 5'- gCAAGCUCAUCGACACCCCAGACAUUAg -3' miRNA: 3'- -GUUCGAGUAGCUGUGGGGUCUGUAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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