Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16681 | 3' | -45.4 | NC_004156.1 | + | 18723 | 0.66 | 0.999999 |
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Target: 5'- -cCGCCCAUagaUGUGAccGUGAGCGa-- -3' miRNA: 3'- uaGCGGGUA---AUAUUucCACUUGUacg -5' |
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| 16681 | 3' | -45.4 | NC_004156.1 | + | 174123 | 0.66 | 0.999998 |
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Target: 5'- uUCGUCCAguuugacUUGUAGuu-UGGACAUGCg -3' miRNA: 3'- uAGCGGGU-------AAUAUUuccACUUGUACG- -5' |
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| 16681 | 3' | -45.4 | NC_004156.1 | + | 81593 | 0.67 | 0.999995 |
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Target: 5'- -aCGCacgguaCCGUUGUAAcguuuGGUGAGCAcgaUGCa -3' miRNA: 3'- uaGCG------GGUAAUAUUu----CCACUUGU---ACG- -5' |
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| 16681 | 3' | -45.4 | NC_004156.1 | + | 142500 | 0.67 | 0.99999 |
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Target: 5'- cUUGCCaCAUcGUGAAGuGUGGGuCAUGUa -3' miRNA: 3'- uAGCGG-GUAaUAUUUC-CACUU-GUACG- -5' |
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| 16681 | 3' | -45.4 | NC_004156.1 | + | 140753 | 0.67 | 0.99999 |
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Target: 5'- uGUCGUCCAUgggaggacGAGGGUGAGUAcGCa -3' miRNA: 3'- -UAGCGGGUAaua-----UUUCCACUUGUaCG- -5' |
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| 16681 | 3' | -45.4 | NC_004156.1 | + | 191967 | 0.69 | 0.99992 |
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Target: 5'- cAUCGCCCGagaaguguuuaccaaUUGUAAA-GUGAACgAUGUg -3' miRNA: 3'- -UAGCGGGU---------------AAUAUUUcCACUUG-UACG- -5' |
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| 16681 | 3' | -45.4 | NC_004156.1 | + | 174674 | 0.71 | 0.998804 |
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Target: 5'- uAUCGCCg--UGUugauGGUGGAUAUGCg -3' miRNA: 3'- -UAGCGGguaAUAuuu-CCACUUGUACG- -5' |
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| 16681 | 3' | -45.4 | NC_004156.1 | + | 126050 | 1.11 | 0.032116 |
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Target: 5'- gAUCGCCCAUUAUAAAGGUGAACAUGCc -3' miRNA: 3'- -UAGCGGGUAAUAUUUCCACUUGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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