Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16682 | 3' | -48 | NC_004156.1 | + | 153514 | 0.66 | 0.999893 |
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Target: 5'- cGGcuAGAUGGUGCGa--GUAGAUGc- -3' miRNA: 3'- -CCuuUCUACCACGUaggCGUCUAUaa -5' |
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| 16682 | 3' | -48 | NC_004156.1 | + | 44796 | 0.66 | 0.999862 |
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Target: 5'- ----uGAUGGUGCAcucggCCGCAaugGAUGUg -3' miRNA: 3'- ccuuuCUACCACGUa----GGCGU---CUAUAa -5' |
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| 16682 | 3' | -48 | NC_004156.1 | + | 13126 | 0.67 | 0.999633 |
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Target: 5'- uGGAaaaAAGAgGGUGCAcUUGCAGAUcgUa -3' miRNA: 3'- -CCU---UUCUaCCACGUaGGCGUCUAuaA- -5' |
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| 16682 | 3' | -48 | NC_004156.1 | + | 39614 | 0.68 | 0.999291 |
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Target: 5'- uGAuAGGUGGUGCAcUCGUgguAGGUGUUg -3' miRNA: 3'- cCUuUCUACCACGUaGGCG---UCUAUAA- -5' |
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| 16682 | 3' | -48 | NC_004156.1 | + | 42106 | 0.7 | 0.994847 |
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Target: 5'- --uGAGAUGGUGCucaaacagaGUCgGCGGAUAa- -3' miRNA: 3'- ccuUUCUACCACG---------UAGgCGUCUAUaa -5' |
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| 16682 | 3' | -48 | NC_004156.1 | + | 130602 | 1.1 | 0.020338 |
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Target: 5'- cGGAAAGAUGGUGCAUCCGCAGAUAUUg -3' miRNA: 3'- -CCUUUCUACCACGUAGGCGUCUAUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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