Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16683 | 3' | -55.4 | NC_004156.1 | + | 68549 | 0.66 | 0.968493 |
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Target: 5'- -gUGUCgagaucaaaagcaaCAGUAGCAaUCCCgGCAACGGu -3' miRNA: 3'- ugACAG--------------GUCGUCGUgAGGG-UGUUGCC- -5' |
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| 16683 | 3' | -55.4 | NC_004156.1 | + | 145652 | 0.66 | 0.963153 |
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Target: 5'- --gGUCCGGCucaGGCGCUCUUugA-CGGc -3' miRNA: 3'- ugaCAGGUCG---UCGUGAGGGugUuGCC- -5' |
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| 16683 | 3' | -55.4 | NC_004156.1 | + | 212273 | 0.66 | 0.9561 |
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Target: 5'- gACUGaaUAGUAGCACUauaCCCACuGCGu -3' miRNA: 3'- -UGACagGUCGUCGUGA---GGGUGuUGCc -5' |
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| 16683 | 3' | -55.4 | NC_004156.1 | + | 95606 | 0.66 | 0.9561 |
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Target: 5'- cGCUGUaCCAGCAcucgaaaucGCuACUgacccucagaCCCACAACGa -3' miRNA: 3'- -UGACA-GGUCGU---------CG-UGA----------GGGUGUUGCc -5' |
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| 16683 | 3' | -55.4 | NC_004156.1 | + | 20720 | 0.68 | 0.894995 |
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Target: 5'- aACUGUCUAGUAGUAgUgCCAaUGAUGGa -3' miRNA: 3'- -UGACAGGUCGUCGUgAgGGU-GUUGCC- -5' |
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| 16683 | 3' | -55.4 | NC_004156.1 | + | 218348 | 0.72 | 0.757784 |
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Target: 5'- cGCaGUUCAGCAaacauGCACUCgCCGCuACGGa -3' miRNA: 3'- -UGaCAGGUCGU-----CGUGAG-GGUGuUGCC- -5' |
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| 16683 | 3' | -55.4 | NC_004156.1 | + | 192931 | 0.72 | 0.738858 |
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Target: 5'- gACUGguggCGGCAGCGCUCaCCACucUGGg -3' miRNA: 3'- -UGACag--GUCGUCGUGAG-GGUGuuGCC- -5' |
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| 16683 | 3' | -55.4 | NC_004156.1 | + | 132803 | 1.09 | 0.00462 |
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Target: 5'- uACUGUCCAGCAGCACUCCCACAACGGu -3' miRNA: 3'- -UGACAGGUCGUCGUGAGGGUGUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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