Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16689 | 3' | -46 | NC_004156.1 | + | 213380 | 0.66 | 0.999997 |
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Target: 5'- uCGAGACUAUuaCAAGAGcGAUGcacACUCu -3' miRNA: 3'- -GCUCUGGUA--GUUCUUaCUACa--UGAGc -5' |
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| 16689 | 3' | -46 | NC_004156.1 | + | 128754 | 0.66 | 0.999997 |
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Target: 5'- aGAcGGCCAUCAAGaAAUGGaGcACUCu -3' miRNA: 3'- gCU-CUGGUAGUUC-UUACUaCaUGAGc -5' |
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| 16689 | 3' | -46 | NC_004156.1 | + | 216743 | 0.68 | 0.99998 |
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Target: 5'- --uGAUCuuGUCGAGAGUGGUGUGCa-- -3' miRNA: 3'- gcuCUGG--UAGUUCUUACUACAUGagc -5' |
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| 16689 | 3' | -46 | NC_004156.1 | + | 169546 | 0.69 | 0.999914 |
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Target: 5'- cCGAGACCgGUCAAcGAGUGccGUACg-- -3' miRNA: 3'- -GCUCUGG-UAGUU-CUUACuaCAUGagc -5' |
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| 16689 | 3' | -46 | NC_004156.1 | + | 206886 | 0.7 | 0.999762 |
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Target: 5'- gGAGGCac-CAcaAAUGGUGUACUCGg -3' miRNA: 3'- gCUCUGguaGUucUUACUACAUGAGC- -5' |
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| 16689 | 3' | -46 | NC_004156.1 | + | 226701 | 0.71 | 0.999267 |
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Target: 5'- gCGcAGACCAcgaaacUCGGGAAgcaucuccuUGAUGUGCUCc -3' miRNA: 3'- -GC-UCUGGU------AGUUCUU---------ACUACAUGAGc -5' |
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| 16689 | 3' | -46 | NC_004156.1 | + | 9516 | 0.71 | 0.999267 |
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Target: 5'- uGAGGCCAaCGAGGAUGAUcaauCUCa -3' miRNA: 3'- gCUCUGGUaGUUCUUACUAcau-GAGc -5' |
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| 16689 | 3' | -46 | NC_004156.1 | + | 193939 | 0.71 | 0.998666 |
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Target: 5'- ---aGCCAUCGAGucuAUGuUGUACUCGg -3' miRNA: 3'- gcucUGGUAGUUCu--UACuACAUGAGC- -5' |
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| 16689 | 3' | -46 | NC_004156.1 | + | 98288 | 0.72 | 0.998388 |
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Target: 5'- uGAGuCCAUCGAGAAUGAaGUG-UCa -3' miRNA: 3'- gCUCuGGUAGUUCUUACUaCAUgAGc -5' |
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| 16689 | 3' | -46 | NC_004156.1 | + | 152485 | 1.12 | 0.029284 |
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Target: 5'- aCGAGACCAUCAAGAAUGAUGUACUCGa -3' miRNA: 3'- -GCUCUGGUAGUUCUUACUACAUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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