Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16691 | 5' | -48.7 | NC_004156.1 | + | 150981 | 0.66 | 0.999856 |
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Target: 5'- -----cCAGUGCAauCGGGCCGAGGAg -3' miRNA: 3'- cagcuaGUCACGUguGUUUGGCUUCU- -5' |
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| 16691 | 5' | -48.7 | NC_004156.1 | + | 220779 | 0.66 | 0.999856 |
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Target: 5'- aGUCGAgaaCGGUGCAaucaacgccaACGAACUGGcAGAg -3' miRNA: 3'- -CAGCUa--GUCACGUg---------UGUUUGGCU-UCU- -5' |
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| 16691 | 5' | -48.7 | NC_004156.1 | + | 85060 | 0.66 | 0.999856 |
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Target: 5'- cUUGggCGGUGUAUACuuGCCGucuAGAc -3' miRNA: 3'- cAGCuaGUCACGUGUGuuUGGCu--UCU- -5' |
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| 16691 | 5' | -48.7 | NC_004156.1 | + | 15722 | 0.66 | 0.999703 |
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Target: 5'- -aCGAUCuAGUGCGCAaguccACCGAAc- -3' miRNA: 3'- caGCUAG-UCACGUGUguu--UGGCUUcu -5' |
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| 16691 | 5' | -48.7 | NC_004156.1 | + | 69778 | 0.67 | 0.99942 |
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Target: 5'- aUUGAUCAGa-UACACGGACCGAuuGGGc -3' miRNA: 3'- cAGCUAGUCacGUGUGUUUGGCU--UCU- -5' |
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| 16691 | 5' | -48.7 | NC_004156.1 | + | 193657 | 0.68 | 0.998931 |
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Target: 5'- -gCGAggAGUGCACGCAAccaucacgaugGCCGAGu- -3' miRNA: 3'- caGCUagUCACGUGUGUU-----------UGGCUUcu -5' |
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| 16691 | 5' | -48.7 | NC_004156.1 | + | 55334 | 0.68 | 0.998703 |
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Target: 5'- aGUgGA-CAGUGUGCGuCAGACUGAAGu -3' miRNA: 3'- -CAgCUaGUCACGUGU-GUUUGGCUUCu -5' |
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| 16691 | 5' | -48.7 | NC_004156.1 | + | 190464 | 0.69 | 0.995671 |
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Target: 5'- -gCGuUCGGUGUAgACGAGuCCGggGAc -3' miRNA: 3'- caGCuAGUCACGUgUGUUU-GGCuuCU- -5' |
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| 16691 | 5' | -48.7 | NC_004156.1 | + | 165851 | 1.09 | 0.020446 |
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Target: 5'- uGUCGAUCAGUGCACACAAACCGAAGAc -3' miRNA: 3'- -CAGCUAGUCACGUGUGUUUGGCUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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