miRNA: 3'- -UGGCcACGUAGUc----UCAGGUGcAAACU- -5'
miRNA: 3'- uGGCCACGUagUCUCAGGUGcaa--ACU- -5'
miRNA: 3'- -UGGCcACGUaGUCUCAGGUGCAaacu -5'
miRNA: 3'- uGGC-CACGUaGUCUCAGGUGCaaacu -5'
miRNA: 3'- -UGGCCACGUAGUCUCAGGUGCAAACU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16692 | 3' | -52.3 | NC_004156.1 | + | 157551 | 0.66 | 0.995226 |
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Target: 5'- uACCGaUGCAUCGuacacGGUCCGCuUUUGAu -3' miRNA: 3'- -UGGCcACGUAGUc----UCAGGUGcAAACU- -5' |
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| 16692 | 3' | -52.3 | NC_004156.1 | + | 143381 | 0.66 | 0.993624 |
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Target: 5'- cCUGGUGCA--AGAGUCCAUagacgUGAa -3' miRNA: 3'- uGGCCACGUagUCUCAGGUGcaa--ACU- -5' |
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| 16692 | 3' | -52.3 | NC_004156.1 | + | 67537 | 0.68 | 0.980454 |
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Target: 5'- uGCUGcUGCAcCAGAGUCCAUGUc--- -3' miRNA: 3'- -UGGCcACGUaGUCUCAGGUGCAaacu -5' |
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| 16692 | 3' | -52.3 | NC_004156.1 | + | 136225 | 0.69 | 0.96741 |
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Target: 5'- cCCGaGUGCA-CGGAGUCUACGa---- -3' miRNA: 3'- uGGC-CACGUaGUCUCAGGUGCaaacu -5' |
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| 16692 | 3' | -52.3 | NC_004156.1 | + | 172615 | 1.09 | 0.009072 |
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Target: 5'- gACCGGUGCAUCAGAGUCCACGUUUGAa -3' miRNA: 3'- -UGGCCACGUAGUCUCAGGUGCAAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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